氨肽酶N在乳腺癌中的表达及与MMP-2、MMP-9 的相关性研究

Aminopeptidase N expression and its correlation with MMP-2, MMP-9 in breast cancer

关键词:  氨肽酶N/CD13  免疫组织化学  乳腺癌  基质金属蛋白酶-2  基质金属蛋白酶-9

Key words:  aminopeptidase N/CD13  immunohistochemistry  breast cancer  matrix metalloproteinase-2  matrix metalloproteinase-9

摘要:

目的　研究氨肽酶N（aminopeptidase N，APN）在乳腺癌中的表达及临床意义，并观察其与基质金属蛋白酶-2（matrix metalloproteinase-2，MMP-2）、基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）的关系。方法　收集河北省人民医院病理科2011年7月至2012年9月乳腺癌石蜡标本60例，癌旁正常乳腺组织石蜡标本20例，及患者的临床病理资料。采用免疫组织化学SP法检测APN在80例标本中的表达，并分析APN表达与乳腺癌患者临床病理特征之间的关系。检测MMP-2、MMP-9在乳腺癌组织中的表达,及与APN表达的相关性。结果　APN在乳腺癌中的阳性表达率36.7%（22/60）明显高于癌旁正常乳腺组织0%（0/20）（χ²=10.115，P=0.001）。APN在乳腺癌中的表达与肿瘤病理类型有关（P=0.035），多表达于浸润性导管癌；与患者绝经状况、肿瘤大小、临床分期、组织学分级、淋巴结转移状态、ER、PR、Her-2等无关（P＞0.05）。乳腺癌组织APN的表达与MMP-2 、MMP-9的表达无相关性（P＞0.05）。结论　APN在乳腺癌中的表达高于正常乳腺组织，但APN在乳腺癌中侵袭转移的机制有待进一步深入研究。

Abstract:

Objective　To investigate the significance of Aminopeptidase N (APN) expression in breast cancer, and to discuss the role of APN in the process of breast carcinoma invasion and metastasis by analyzing the relationships between APN and Matrix metalloproteinase-2 (MMP-2), Matrix metalloproteinase-9 (MMP-9). Methods　Immunohistochemistry (SP method) was performed to detect the expressions of APN, MMP-2 and MMP-9 in 60 cases of breast cancer tissues, as well as APN expression in 20 cases of adjacent normal breast tissues. Results　The positive frequency of APN expression in breast cancer tissues (36.7%, 22/60) was significantly higher than that in adjacent normal breast tissues (0%, 0/20) (χ²=10.115, P=0.001). APN expression was associated with tumor pathological types (P=0.035). However, there was no relationship between the APN expression and other clinicopathological features, such as menopausal state, clinical stage, tumor size, histological grade, nodal status, ER, PR, and Her-2 (P＞0.05). There was no significant relationship between the APN expression and MMP-2, MMP-9 expression (P＞0.05). Conclusion　APN expression was higher in breast cancer than that in normal breast tissues, but it needs further study if APN expression could play a role in invasion and metastasis in breast cancer.