扁蒴藤素对人乳腺癌MCF-7细胞生长和凋亡的影响及机制研究

Effect of pristimerin on the growth and apoptosis of MCF-7 breast cancer cells and the mechanism

关键词:  扁蒴藤素  乳腺癌  MCF-7  凋亡

Key words:  pristimerin  breast cancer  MCF-7  apoptosis

摘要:

目的　研究扁蒴藤素对乳腺癌细胞MCF-7生长和凋亡的影响及其机制。 方法　用MTT法检测扁蒴藤素对MCF-7细胞细胞生长的影响；用Annexin V-FITC/PI双染及TUNEL染色检测扁蒴藤素对MCF-7凋亡的诱导作用；用western blot分析扁蒴藤素作用后，凋亡相关因子Bcl-2和Bax蛋白的表达变化。 结果　扁蒴藤素对MCF-7细胞的生长有显著的抑制作用，对其作用48 h的半数抑制浓度（IC₅₀）为0.59 μmol/L。1.0 μmol/L扁蒴藤素作用后,Annexin V-FITC/PI染色凋亡细胞比率（15.55±1.43)%，TUNEL阳性的MCF-7细胞比率为（35.59±4.43）%，较对照组（未经扁朔藤素作用）［（1.45±0.24）%和（0.85±0.34）%］明显升高，差异均有显著性意义，P<0.05。Western blot结果显示，扁蒴藤素能下调Bcl-2的表达，上调Bax的表达。 结论　扁蒴藤素素通过调节细胞凋亡相关因子诱导MCF-7细胞凋亡的发生，从而高效抑制MCF-7乳腺癌细胞的生长。

Abstract:

Objective　To investigate the effect of pristimerin on the growth and apoptosis of MCF-7 breast cancer cells and to explore the underlying mechanism. Methods　The effect of pristimerin on the growth and of apoptosis MCF-7 cells were analyzed by MTT assay and Annexin V-FITC/PI staining, TUNEL staining, respectively. The expression of Bcl-2 and Bax was determined by western blotting analysis. Results　Pristimerin exhibited a marked inhibition on the survival of MCF-7 cells, and the half maximal inhibitory concentration (IC₅₀) value was 0.59 μmol/L. The numbers of apoptotic MCF-7 cells, as revealed by Annexin V binding and TUNEL assay, increased upon pristimerin treatment. Pristimerin treatment resulted in a decrease of Bcl-2 and an increase of Bax expression. Conclusion　Our results demonstrated that pristimerin suppressed the proliferation of MCF-7 cells, and that these effects occurred through increasing apoptosis by regulation of apoptotic effectors.