慢性结石性胆囊炎胆汁胆固醇浓度与胆囊收缩素受体的特点及关系

Correlation of cholecystokinin receptor expression and biliary cholesterol concentration in chronic calculous cholecystitis

关键词:  胆汁胆固醇  胆囊收缩素受体  慢性结石性胆囊炎

Key words:  biliary cholesterol  cholecystokinin receptor  chronic calculous cholecystitis

摘要:

目的　通过检测慢性结石性胆囊炎患者胆囊胆汁胆固醇浓度(biliary cholesterol concentration，BCC)及胆囊黏膜上皮A型胆固醇受体(cholecystokinin-A receptor，CCK-AR)蛋白表达强度，明确BCC与CCK-AR的特点及关系。方法　通过免疫组化的方法检测34例行腹腔镜手术的慢性结石性胆囊炎胆囊黏膜上皮CCK-AR蛋白表达强度，同时用生化方法检测胆囊内胆汁胆固醇浓度，观察结果特点并分析相关性。结果　所有标本胆囊黏膜上皮CCK-AR呈阳性表达，其中13例呈弱阳性表达，9例呈中阳性表达，12例呈强阳性表达。胆囊内胆汁胆固醇浓度在CCK-AR弱阳性组，CCK-AR中阳性组，CCK-AR强阳性组分别为(144±51)mmol/L，(168±41)mmol/L和(96±19)mmol/L，与CCK-AR表达强度呈负相关(r=-0.702，P=0.000)。结论〖HTK〗　胆汁胆固醇浓度可作为评估胆囊黏膜上皮CCK-AR分子蛋白表达的潜在指标。

Abstract:

Objective To investigate the relationship of biliary cholesterol concentration and cholesterol cholecystokinin-A receptor expression in chronic calculous cholecystitis.Methods The biliary cholesterol concentration in the gallbladder of4 cases were tested by biochemistry and cholesterol cholecystokinin-A receptor expression in the gallbladder mucosa of4 cases were detected by immunohistochemistry.Results The cholesterol cholecystokinin-A receptor (CCK-AR) expression in the gallbladder mucosa of4 cases showed positive expression，in which weak positive3 cases，moderate positive 9 cases，and strong positive2 cases.Bile cholesterol concentration in the weak positive group was (144±51) mmol/L，in the moderate positive group was (168±41) mmol/ L，and in the strong positive group was (96±19) mmol/L.The CCK-AR expression and biliary cholesterol concentration showed negative correlation (r=-0.702，P=0.000).Conclusion The bile cholesterol concentration may be used as potential indicators of CCK-AR expression in chronic calculous cholecystitis，providing a theoretical basis for further study of the gallbladder dynamics.