| 摘要: |
| 目的 检测单羧酸转运体1（MCT1）在鼻咽癌病人和正常人外周血髓系抑制性细胞（MDSC）中的表达并探讨其临床意义。方法 随机收集经病理确诊的鼻咽癌病人和正常人外周血各10例，红细胞裂解液处理后，采用BD FACSAria Ⅲ流式细胞仪分选出MDSC（CD11b+CD33+HLA-DR-），q-PCR检测比较两组MCT1 mRNA水平的差异。结果 鼻咽癌病人外周血中MDSC的比例（19.77±77）%显著高于正常人（0.84±0.18）%，P<0.001；且前者MCT1 mRNA水平（05±0.56）为后者（15±0.33）的88倍，差异有显著性意义(P<0.01）。结论 鼻咽癌病人外周血中的MDSC可能是凭借其在数量上的优势及能量代谢上的特性，更好地发挥免疫抑制效应。 |
| 关键词: MCT1 MDSC 鼻咽癌；能量代谢；免疫抑制 |
| DOI：10.11724/jdmu.2016.02.03 |
| 分类号:R739.62; R739.63 |
| 基金项目:基金项目:福建省自然科学基金项目（2014J05088） |
|
| Expression and significance of MCT1 in MDSC from peripheral blood of nasopharyngeal carcinoma patients |
|
ZHANG Na1, LAN Rui-long2, ZHONG Guang-xian3
|
|
1.Department of Hematology，Fujian Medical University Union Hospital，Fujian Insitute of Hematology，Fujian Provincial Key Laboratory of Hematology，Fuzhou50001，China;2.Department of Central Laboratory，the First Affiliated Hospital of Fujian Medical University，Fuzhou50005，China;3.Department of Orthopaedics，the First Affiliated Hospital of Fujian Medical University，Fuzhou50005，China
|
| Abstract: |
| Objective To investigate MCT1 expression of MDSC in peripheral blood obtained from nasopharyngeal carcinoma (NPC) patients and healthy donors，and discuss its clinical significance.Methods Peripheral blood samples were obtained randomly from0 NPC patients and0 healthy donors，processed by RBC lysates and MDSC (CD11b+CD33+HLA-DR-) within them were sorted by BD FACSAriaⅢ.Quantitative rt-PCR were adopt to determine the MCT1 mRNA levels，t-test was used to evaluate the significance between groups.Results The MDSC ratio of NPC patients (19.77±77)% was significant higher than that of healthy donors (0.84±0.18)% (P<0.001).MCT1 mRNA level (05±0.56) of MDSC obtained from NPC patients was significantly higher than that of healthy donors (15±0.33) (P<0.01).Conclusion MDSC within peripheral blood of NPC patients could play an immunosuppressive effect by its superiority in number and energy metabolism property. |
| Key words: MCT1 MDSC nasopharyngeal carcinoma energy metabolism immunosuppression |
