种植体周围龈沟液中TNF-α和MMP-8水平的研究

Levels of TNF-α and MMP-8 in peri-implant sulcular fluid

关键词:  种植体周围炎  肿瘤坏死因子-α  基质金属蛋白酶-8

Key words:  peri-implantitis  TNF-α  MMP-8

摘要:

目的　探讨种植体周围龈沟液中肿瘤坏死因子-α（TNF-α）和基质金属蛋白酶-8（MMP-8）的水平与种植体周围炎的关系。　方法　选择2012年5月至2014年5月在大连医科大学口腔医学院行OSSTEM种植体种植修复的患者36例作为研究对象（种植体48颗）,根据种植体周围情况将其分为健康种植体组（38颗）和炎症种植体组（10颗），将对侧同名健康天然牙作为对照组（48颗）。收集各组种植体和天然牙周围龈沟液，运用ELISA法检测其中TNF-α和MMP-8的浓度，同时检测牙周探诊深度（PD）和牙龈出血指数（SBI）。分析PD、SBI值与龈沟液中TNF-α、MMP-8水平的相关性。　结果　炎症种植体组PD、SBI以及龈沟液中TNF-α和MMP-8水平均高于健康种植体组和健康天然牙组，差异具有显著性意义（P<0.05）。SBI、PD与TNF-α、MMP-8水平均呈正相关关系（P<0.01）。　结论　种植体周围龈沟液中TNF-α和MMP-8水平和种植体周围炎密切相关，可在一定程度上反映种植体周围组织的健康状态。

Abstract:

Objective To investigate the relation of TNF-α and MMP-8 levels in peri-implant sulcular fluid with peri-implantitis. Methods 36 patients were enrolled who received oral implant treatment at the College of Stomatology of Dalian Medical University. All the implants （n=48) were divided into health implant group（n=38）and inflammation implant group（n=10），and 48 healthy natural teeth were collected as the control group. Gingival crevicular fluid was sampled and levels of TNF-α and MMP-8，PD and SBI were measured and analyzed. Results PD, SBI and the levels of TNF-α and MMP-8 of inflammation implant group were higher than healthy natural teeth group and healthy implant group（P<0.05）. PD and SBI had positive correlation with the levels of TNF-α and MMP-8（P<0.01）. Conclusion The levels of TNF-α and MMP-8 in gingival crevicular fluid have close relationship with peri-implantitis，which can reflect the health condition of peri-implant tissues to some extent.