CeO2的制备、表征及对PC12细胞氧化损伤的保护作用

Preparation, characterization of nanoceria and its protection on oxidative damage of PC12 cells

关键词:  CeO₂  制备  PC12细胞  抗氧化损伤

Key words:  nanoceria  preparation  PC12 cells  antioxidative damage

摘要:

目的　制备纳米氧化铈（nanoceria，CeO₂），初步探讨其对肾上腺嗜铬细胞瘤细胞PC12氧化损伤的保护作用。　方法　采用均相沉淀法制备纳米CeO₂，对其晶粒大小和晶粒形貌进行表征。将粗浓度为250 mmol/L的CeO₂加入PC12细胞培养液，通过20 μmol/L H₂O₂诱导细胞损伤，建立PC12细胞的氧化应激损伤模型，采用MTT法检测对照组、CeO₂组、CeO₂+H₂O₂组，H₂O₂组的细胞活性，测定CeO₂对PC12细胞的保护作用。　结果　采用均相沉淀的方法合成粒子直径为5~10 nm的CeO₂ 。 MTT实验显示H2O2组细胞存活率为（74.61±3.97）%，CeO₂ +H₂O₂组的细胞存活率为（99.21±4.01）%，明显高于H2O2组（P<0.05）。　结论　CeO₂对H₂O₂造成的氧化损伤细胞具有保护作用。

Abstract:

Objective To prepare nanoceria (CeO₂) and to investigate the protective effect on oxidative damage of Pheochromocytoma cell (PC12). Methods Homogenous precipitation method was used to prepare the nanoceria. XRD and TEM were used to characterize and determine the size of CeO₂. 250 mmol/L rough concentration of CeO₂ was added into PC12 cell culture fluid. Cells were induced and damaged by 20 μmol/L H₂O₂. And oxidative stress damage model of PC12 cell was set up. The cell survival rate of control group, CeO₂+H₂O group and H₂O₂group was measured by MTT method, respectively. The antioxidative protection of CeO₂ to the PC12 damaged by H₂O₂ was evaluated. Results The size of nanoceria was 5-10 nm. According to the results of MTT method, it was shown that the cell survival rate of H₂O₂ group was (74.61±3.97)%. And the cell survival rate of CeO₂+H₂O group was (99.21±4.01)%, which was significantly higher than H₂O₂ group (P＜0.05). Conclusion Nanoceria would have the protective effect on the PC12 which was oxidative damaged by H₂O₂.