循环肿瘤细胞检测在不同转移灶晚期乳腺癌中的应用研究

Detection of circulating tumor cells in advanced breast cancers with different metastases

关键词:  乳腺癌  循环肿瘤细胞  单发转移

Key words:  [Key words]　breast cancer  circulating tumor cell  metastasis

摘要:

[摘要]　目的　探讨不同单发转移部位乳腺癌患者对循环肿瘤细胞（circulating tumor cell，CTC）检测的敏感性。　方法　采集119例乳腺癌单发转移（肝、肺、骨）患者的外周血7.5 mL，按转移部位分成3组，采用cellsearch方法检测血液标本中的CTC，统计分析各组检出率及阳性率，并比较其差异。　结果　晚期乳腺癌肝、肺、骨转移患者外周血中CTC的检出率分别为35.5%（11/31）、47.5%（19/40）、68.8%（33/48），骨转移组的检出率明显高于肺及肝转移组（P<0.05）。肝、肺、骨转移组CTC的阳性率分别为32.3%（10/31）、12.5%（5/40）、12.5%（6/48），肝转移组与肺及骨转移组比较差异有显著性意义（P<0.05）。　结论　CTC检测对于晚期乳腺癌骨转移患者的敏感性较高。相比骨转移及肺转移，晚期乳腺癌肝转移患者的预后相对较差。

Abstract:

[Abstract] Objective To evaluate the detection sensitivities and prognosis of advanced breast cancers with different metastases by detecting circulating tumor cells (CTC). Methods Blood samples (7.5 mL) were obtained from 119 patients of advanced breast cancer with different metastases (liver, lung, bone). Patients were divided into liver group, lung group, and bone group accordingly. Cell search system was used to detect CTC of all samples. The results were analyzed using statistical methods. Results The CTC detection rate in blood samples was 35.5%（11/31） in liver group, 47.5%（19/40） in lung group and 68.8%（33/48） in bone group, respectively. The rate in bone group was significantly higher than those in the other two groups. The positive incidence of CTC detection in liver group was significant higher than those in lung and bone groups. Conclusion CTC detection is more suitable for patients of advanced breast cancer with bone metastasis because of a higher sensitivity. A poor prognosis of liver metastasis patients could be predicted by CTC detection.