姜黄素PLGA-TPGS纳米粒在小鼠体内的药效学研究

Study on the in vivo pharmacodynamics in mice of curcumin-loaded PLGA-TPGS Nanoparticles

关键词:  姜黄素  纳米粒  HCa-F细胞  实体瘤

Key words:  [Key words]　curcumin  nanoparticles  HCa-F cells  solid tumors

摘要:

[摘要]　目的　考察姜黄素PLGA-TPGS纳米粒（curcumin-loaded PLGA-TPGS nanoparticles，CPTN）体内对荷小鼠腹水型肝癌高淋巴道转移细胞HCa-F实体瘤的抑制作用。　方法　建立荷HCa-F细胞的小鼠实体瘤模型，尾静脉分别注射生理盐水、空白纳米粒、姜黄素溶液、5-氟尿嘧啶溶液和CPTN，7 d后取出实体瘤，测量瘤径、瘤重、测定瘤内药量并进行HE染色，全面评价CPTN对肝癌实体瘤的治疗效果。　结果　体内药效学实验中CPTN组和5-氟尿嘧啶溶液组的瘤体体积增长量和瘤重明显低于生理盐水（空白对照）组，差异具有显著性意义（P<0.05）；两组抑瘤率分别为63.44%和38.14%。姜黄素溶液组和CPTN组实体瘤中的姜黄素量分别为（3.75±0.22）μg和（31.64±2.54）μg，差异具有显著性意义（P<0.01）。HE染色后镜下观察该组切片显示大部分细胞丢失细胞核，破碎成碎片，边缘不完整，表明其治疗肿瘤效果最佳。　结论　CPTN在体内对荷HCa-F细胞的小鼠实体瘤有一定的抑制作用，且治疗效果好于5-氟尿嘧啶注射液。

Abstract:

[Abstract]　Objective　To investigate the in vivo therapeutic effects on ascitic hepatocarcinoma cell strain with high metastasis potential in lymphatic system (HCa-F) solid tumors in mice with curcumin (CM)-loaded polylactic-co-glycolic-D-α-tocopherol polyethylene glycol 1000 succinate acid nanoparticles (CPTN). 　Methods　HCa-F cells were inoculated into mice's armpit to form of liver cancer solid tumor model. Drug was intravenously injected by normal saline,empty PTN,curcumin solutions (CS),fluorouracil solutions (FS) and CPTN respectively once a day for consecutive seven days. After the treatment,solid tumors were taken,measured diameter and weighed,determine CM mass in tumors,embedded in paraffin and sectioned. The slices were stained by HE to comprehensively evaluate the combination therapy of CPTN for the treatment of solid tumors.　Results　For 7 days treatment, tumor volume growth of CPTN group and FS group were significantly slower than the normal saline (blank control) group (P<0.05), CM mass of CPTN group were significantly higher than that of CS group (P<0.01). Inhibition rate of the CPTN group could reach more than 60%, and the slice stained with HE of CPTN group shows that almost all cells lost nuclei and broke into fragments with fuzzy edge. The results indicated CPTN got the best effect against liver cancer.　Conclusions　Results of pharmacodynamic tests in vivo showed that CPTN had a good effect for liver cancer,and treatment of the mice in CPTN group was better than FS group.