| 引用本文: | 姚晓峰 1,2，孙 睿 1,2，姜丽平 1,2，耿成燕 1,2，仲来福 1,2，郑白璐 1,2，杨 光 1，刘 爽 1，孙鲜策 1,2.亚砷酸钠对胰岛细胞中p53、mdm2和Kras基因及蛋白表达的影响[J].大连医科大学学报,2014,36(6):518-521. |
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| 摘要: |
| [摘要] 目的 探讨砷对大鼠胰岛β细胞（INS-1）p53、mdm2和Kras基因和蛋白表达的影响。 方法 荧光实时定量PCR法检测亚砷酸钠对大鼠胰岛β细胞中野生型p53（Tp53）、mdm2和Kras基因表达的影响，Western blot检测亚砷酸钠对大鼠胰岛β细胞突变型p53和mdm2蛋白表达的影响。 结果 亚砷酸钠作用于INS-1细胞后，Tp53基因水平降低，mdm2、Kras基因水平升高；突变型p53和mdm2蛋白表达增加。 结论 亚砷酸钠可使INS-1细胞中抑癌基因Tp53向突变型p53转变，癌基因mdm2和Kras的水平升高。 |
| 关键词: 亚砷酸钠 大鼠胰岛β细胞 p53 mdm2 Kras |
| DOI：10.11724/jdmu.2014.06.02 |
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| 基金项目:基金项目：国家自然科学基金项目 (30972562)；辽宁省自然科学基金项目（2014023050） |
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| Effect of sodium arsenite on the gene and protein expression of p53, mdm2 and Kras in islet cells |
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YAO Xiao-feng 1,2, SUN Rui 1,2, JIANG Li-ping 1,2，GENG Cheng-yan 1,2，ZHONG Lai-fu 1,2，ZHENG Bai-lu 1,2，YANG Guang 1，LIU Shuang 1, SUN Xian-ce 1,21,2
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1.Department of Occupational and Environmental Health, Dalian Medical University, Dalian 116044, China;2.Liaoning Anti- Degenerative Diseases Natural Products Engineering Research Center，Dalian Medical University, Dalian 116044, China
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| Abstract: |
| [Abstract] Objective To investigate the effect of sodium arsenite on p53, mdm2 and Kras expressions in islet β cells (INS-1) of rat. Methods The levels of wild type p53 (Tp53), mdm2 and Kras in sodium arsenite-treated rat islet β cells were detected by real-time PCR. The expression of mutant p53 and mdm2 protein were detected by western blot. Results After treatment with sodium arsenite, the level of Tp53 decreased, but those of mdm2、Kras increased. The protein expressions of mutant p53 and mdm2 increased. Conclusion Sodium arsenite could induce the transformation of Tp53 to mutant p53, and increase the level of mdm2 and Kras in INS-1 cells. |
| Key words: [Key words] sodium arsenite rat pancreatic β cell p53 mdm2 Kras |
