乌司他丁对老年小鼠术后中枢炎症反应及学习记忆能力的影响

Effects of ulinastatin on memory and inflammation in hippocampus after opereiting in aged mice

关键词:  记忆  炎症  手术  乌司他丁

Key words:  [Key words] memory  inflammation  surgery  ulinastatin

摘要:

[摘要]　目的　探索使用乌司他丁对老年小鼠术后中枢炎症反应及术后学习记忆能力的影响。　方法　老年小鼠随机分为对照组（C组），手术组（S组），乌司他丁组（W组），S组和W组建立小鼠剖腹探查模型，W组于手术前10 min腹腔注射乌司他丁（10 000 μ/mL,100 μ/g），S组于手术前10 min注射生理盐水0.5 mL。术后1 d,3 d,7 d采用real-time PCR检测各组小鼠海马IL-1β、TNF-α浓度，使用水迷宫实验检测小鼠术后7 d学习记忆能力。　结果　与对照组C组相比，S组，W组术后1 d,3 d,7 d小鼠海马IL-1β,TNF-α表达均增多；术后1 d、3 d,W组表达较S组较低。与C组比较，S组，W组小鼠水迷宫术后4~6 d潜伏期延长，术后7 d穿越原平台次数减少，但W组较S组术后3~6 d潜伏期短，术后7 d穿越原平台次数多。　结论　乌司他丁可抑制手术后老年小鼠中枢炎症反应，改善小鼠手术引起的学习记忆能力。

Abstract:

[Abstract] Objective To investigate using ulinastatin to the change in aged mouses'memory and central nervous inflammaorg reartion fillouwing sallgical operation. 　Methods Aged mouses were randomly allocated to the following na ve(group C), abdominal surgery alone(group S) and intraperitoneally injection of ulinastatin before surgery(group U). Hippocampal IL-1β,TNF-α were measured by real-time PCR 1 d,3 d,7 d after surgery.Mouse’memory was assessed in Morris water maze test. 　Results Compared with that of control, the expression of IL-1β,TNF-αin mouse’ hippocampa are enhanced after surgery and diminished if using ulinastatin 1 d,3 d or 7 d after surgery. Surgery could result in mouse'memory impairment and diminished if using ulinastatin in Morris water maze. 　Conclusion Ulinastatin could suppoess cerebral inflammating reastron and diminish the memory impairment induced by operctron in mice.