微卫星vWA和D5S818与精神分裂症的多态性分析

Relationship between polymorphism of microsatellite vWA and D5S818 with schizophrenia

关键词:  微卫星DNA  精神分裂症  第12号染色体

Key words:  [Key words] microsatellite DNA  schizophrenia  chromosome 12

摘要:

[摘要] 目的探讨微卫星vWA和D5S818的多态性与精神分裂症有关的易感基因区域。方法　对57例精神分裂症患者（精神分裂症组）和123名随机人群（对照组）采用PE Profiler plus系统进行PCR复合扩增，然后用ABI公司的310型基因分析仪自动进行电泳分离和基因检测。结果　精神分裂症组的vWA-14 的检出率为21.9 %，对照组检出率为33.3 %，两者之间差异有显著性意义（P<0.05）；D5S818位点的各等位基因频率的检出率两组之间差异无显著性意义（P>0.05）。经吻合度检验，2个微卫星各等位基因频率的分布，均符合Hardy-Weinberg平衡。结论　vWA-14位点与精神分裂症有明显关联，提示vWA-14 可能与精神分裂症某种抵抗基因连锁，表明在第12号染色体上存在与精神分裂症相关的基因。

Abstract:

[Abstract] Objective To explore a genetic relationship between the polymorphism of microsatellites vWA and D5S818 with schizophrenia. Methods DNA samples collected from patients with schizophrenia (n=57) and random population (n=123) were amplified by PE Profiler plus system for multiplex PCR. Then ABI 3130-Avent genetic analyzer was used for automatic capillary electrophoresis and genotyping. Results The detection ratios of vWA-14 were 21.9 % and 33.3 % in schizophrenia group and control group, respectively. The difference was statistically significant between the two groups (P<0.05). While there was no significant difference in the frequency of D5S818 between the groups (P>0.05). No deviations from the Hardy-Weinberg equilibrium were observed. Conclusion There is a strong association of vWA-14 with schizophrenia, suggesting a possible linkage between vWA-14 and some schizophrenia resistant genes. There may be genes on chromosome 12 associated with susceptibility to schizophrenia.