大鼠脑积水及其分流模型建立

1. Department of Neurosurgery, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;2. Department of Pathophysiology, Dalian Medical University, Dalian 116044,China

关键词:  大鼠  脑积水  分流  模型

Key words:  rat  hydrocephalus  shunt  model

摘要:

目的　建立大鼠脑积水及脑积水分流模型。方法　雄性Wistar大鼠80只，随机分为实验组和对照组各40只，实验组大鼠应用显微外科技术，在显微镜下向枕大池中注射250 mg/mL高岭土混悬液0.05 mL，对照组用同样方法向枕大池注入生理盐水0.05 mL，34只完成脑积水模型的实验组大鼠，再根据观察时间随机分成A（3d）、B（1周）、C（3周）、D（3周脑积水模型分流术后3 d）、E（3周脑积水模型分流术后1周）组，35只完成脑积水对照组大鼠，随机再根据观察时间分为A1（3d）、B1（1周）、C1（3周）、D1（3周脑积水模型分流术后3 d）、E1（3周脑积水模型分流术后1周）组。D、E组在显微镜下分别行侧脑室-皮下分流术， D1、E1组大鼠用和D、E组大鼠相同的方法，只是不放置分流管，通过脑室病理切片, MR观察脑室系统的变化。结果　实验组大鼠脑室随着时间的延长而逐渐扩大，脑积水症状逐渐加重，分流后脑室随着时间的延长而逐渐缩小，症状得到改善。脑织织切片脑室指数测定：A组为0.165±0.076，B组0.293±0.096，C组0.487±0.133；对照组各亚组各对应时间点差异无显著性意义（P>0.05），平均为0.029±0.010。A、B、C组和A1、B1、C1组比较均P<0.001，A、B、C组间比较P<0.05。D组侧脑室指数0.322±0.054，E组0.031±0.012，分别与D1、E1组比较，D、E组间比较均P<0.001。结论　高岭土枕大池注射法制作的大鼠交通性脑积水模型及脑积水脑室-皮下分流术模型，适用于脑积水发病机理及分流术后脑组织病理改变的实验研究，对脑积水病理机制和治疗方法研究有意义。

Abstract:

Objective 　To establish a rat hydrocephalus and hydrocephalus shunt model. Methods Eighty Male Wistar rats Were randomly divided into experimental and control groups of 40 each, Experimental rats injected to cisterna magna with 250 mg/mL kaolin suspension 0.05 mL by microsurgical techniques under the microscope,The same way, the control group injected with saline 0.05 mL to cisterna magna, completed the model of hydrocephalus in 34 rats as the experimental group 1, and then randomly divided into A, B, C, D, E group, completed control in 35 rats as the control group 1, and then randomly divided into A1, B1, C1, D1, E1 group, D, E groups were performed under a microscope the lateral ventricles-subcutaneous shunt, the same way, D1, E1 group does not place the shunt tube, The changes was observed in the ventricular system by Ventricle Slice, MR. Results Ventricle index determination in Brain tissue slices: A group 0.165±0.076,B group 0.293±0.096, C group 0.487±0.133; Subgroups of the control group showed no significant difference at each time point（P>0.05），An average of 0.029 ± 0.001. Comparison between A、B、C and A1、B1、C1 groups, P< 0.001, Comparison between A,B,C groups , P< 0.05. D group lateral ventricle index 0.322±0.054，E group 0.031±0.012, Comparison between D,E and D1,E1 groups, P<0.001. Conclusion The rats communicating hydrocephalus model and hydrocephalus ventricle-subcutaneous shunt model by Kaolin cisterna magna injection, Suitable for hydrocephalus pathogenesis and experimental study of the pathological changes of brain tissue after shunt, It is meaningful for Pathological mechanisms and research of hydrocephalus treatment.