慢性酒精中毒性肌病伴随免疫状态变化的实验研究

Immune function study of chronic alcoholic myopathy

关键词:  酒精  肌病  B细胞  免疫

Key words:  alcohol  myopathy  B cells  immune

摘要:

目的　通过对慢性酒精中毒性肌病大鼠模型的免疫功能变化研究，探讨慢性酒精中毒性肌病发生时机体免疫功能的状态。　方法　构建SD大鼠慢性酒精中毒性肌病模型，同时设立对照组，对两组大鼠进行卵清蛋白抗原注射免疫，用ELISA法连续检测大鼠血清抗卵清蛋白抗体的变化，同时用流式细胞仪检测两组大鼠CD19阳性B细胞百分率。　结果　造模完成时，实验组大鼠体重与对照组相比，差异有显著性意义（P<0.05），HE染色与透射电镜结果显示实验组大鼠跖肌II型肌纤维受累明显，呈现慢性酒精中毒性肌病典型变化。随着慢性酒精中毒性肌病大鼠模型的逐渐建立，实验组大鼠体内抗卵清蛋白抗体效价持续下降，在7个检测点内与对照组大鼠相比，差异均有显著性意义（P<0.05）。同时，实验组大鼠CD19阳性的B细胞百分比也呈现显著下降的趋势，与对照组大鼠相比，差异有显著性意义（P<0.05）。　结论　在慢性酒精中毒性肌病大鼠模型机体中B细胞百分比呈现下降趋势，慢性酒精中毒性肌病发生时会同时伴有体液免疫功能低下情况发生。

Abstract:

[Abstract] Objective To study the relationship between chronic alcoholic myopathy and immune function. 　Methods SD rats chronic alcoholic myopathy model were constructed. A control group SD rats were studied at the same time. The two groups of rats were injected with ovalbumin antigen. The anti-ovalbumin antibody was detected with ELISA method. At the same time, CD19 positive B cell percentage was detected with flow cytometry. 　Results At the end of the modeling, the body weight of the experimental group was decrease compared with the control group. The difference was significant (P<0.05) between the two groups. HE staining and transmission electron microscopy results showed that type II muscle fibers of plantaris in the experimental group rats typically changed as the chronic alcoholtoxic myopathy. When the chronic alcoholic myopathy occurs, the antibody titer of anti-ovalbumin continued to decline. In seven control point, there were significant differences (P<0.05) compared with the control group. At the same time, the percentage of CD19 positive B cell was also reduced. The difference was significant (P<0.05) between the two group rats. 　Conclusion When chronic alcoholic myopathy happens, the percentage of B cell is decline, the humoral immunity function will be affected at the same time.〖JP〗