大承气汤对急性坏死性胰腺炎肠道推进功能变化的影响

Effect of Da Cheng Qi decoction to the intestinal transtit of acute necrotizing pancreatitis (ANP) in rat

关键词:  胰腺炎；急性坏死性；胃肠通过试验  大承气汤

Key words:  pancreatitis  acute necrotizing  gastrointestinal transit  DaChengQi decoction

摘要:

[目的] 研究急性坏死性胰腺炎时肠道推进功能变化和大承气汤的影响。[方法]SD大鼠制成急性坏死性胰腺炎检测其肠道推进功能的模型，分成假手术组、模型组、中药治疗组。模型建立48 h后从十二指肠注入荧光标记右旋糖酐10 000，25 min后处死动物，各组检测10等分段小肠荧光标记物的含量，计算其几何中心；取盲肠内容物进行细菌培养。[结果]荧光标记物平均几何中心在小肠的位置，模型组较假手术组显著落后（P<0.01），中药治疗组较模型组显著提前（P<0.01）；盲肠内容物培养菌量模型组需氧菌总数和革兰阴性菌菌数较假手术组显著增加(P<0.01),中药治疗组较模型组显著降低（P<0.01）。[结论]急性坏死性胰腺炎肠道推进功能减弱，大承气汤可明显的提高肠推进功能。

Abstract:

[Objective] To investigate the the effect of Da Cheng Qi decoction to the intestinal transtit of acute necrotizing pancreatitis (ANP) in rat . [Methods] The modols of testing the intestinal transit of ANP in rat were established, and SD rat were divided in to sham operation group, model group and treatment group. After 48 hr, FITC dextran 10000 were injected into the duodenum, 25 min later, the levels of FITC dextran 10000 in 10-equal-part-intestine were tested, and the average geometric centre were calculated. [Results] The average geometric center of the fluorescent marker model group than the sham operation group were significantly more backward（P<0.01）, treatment group compared with the model group significantly more ahead（P<0.01）. Cecal contents of the culture to a significant increase in the total number of bacteria quantity model group aerobic and gram-negative bacteria, bacterial counts than the sham operation group group (P<0.01), the treatment group significantly reduced compared with the model group（P<0.01）.[Conclusions] There was decreased intestinal transtit in acute necrotizing pancreatitis. Da Cheng Qi decoction could increased intestinal transit.