TSHR和MMP-9在甲状腺乳头状癌组织中的表达及其临床意义

Clinical significances of the TSHR and MMP-9 expressions in papillary thyroid carcinoma

关键词:  甲状腺乳头状癌  TSHR  MMP-9  免疫组织化学法

Key words:  papillary thyroid carcinoma  TSHR  MMP-9  immunohistochemistry

摘要:

[目的] 探讨甲状腺乳头状癌(PTC)组织中促甲状腺激素受体(TSHR)与基质金属蛋白酶（MMP-9）的表达及其与病理参数之间的关系。[方法]应用免疫组织化学(SP)法检测56例PTC，30例结节性甲状腺肿及11例正常甲状腺组织标本的TSHR和MMP-9蛋白表达。[结果]TSHR蛋白在PTC、结节性甲状腺肿和正常甲状腺组织的表达率分别为91.07％、70.00 %和27.27 %，三组间比较差异有显著性意义(P<0.05)；TSHR在有淋巴结转移PTC组与无淋巴结转移组的表达率分别为76.92 %与95.35 %( P>0.05)；在Ⅲ～Ⅳ期与Ⅰ～Ⅱ期的表达率分别为89.66%与92.60% (P>0.05)。 MMP-9蛋白在PTC、结甲和正常甲状腺组织中表达率分别为88.37％、36.67 %和 0 %，三组间比较差异有显著性意义(P<0.05)；在有淋巴结转移PTC组与无淋巴结转移PTC组的表达率分别为92.31 %与79.07%(P>0.05)，在Ⅲ～Ⅳ期与Ⅰ～Ⅱ期的表达率分别为89.66 %与74.07 %(P>0.05)。TSHR、 MMP-9的表达与患者的性别、年龄及肿瘤大小均无相关性(P>0.05)。[结论]TSHR蛋白高表达与甲状腺乳头状癌的发生有关，可能是甲状腺癌发生的一个早期标志；MMP-9高表达可能与甲状腺癌细胞侵袭和转移密切相关。

Abstract:

[Objective] To evaluate the expressions of thyroid stimulating hormone receptor (TSHR) and matrix metalloproteinase-9(MMP-9) in papillary thyroid carcinoma（PTC) and their clinical significances. [Method] The SP immunohistochemistry was used to detect the expressions of TSHR and MMP-9 protein in 56 cases of PTC, 30 cases of nodular goiter and 11 cases of normal thyroid tissue. [Results] The positive expression rate of TSHR in PTC was significantly higher than those of nodular goiter and normal thyroid tissue(P<0.05). and the rates in PTC with lymph node metastasis and without were 76.92% and 95.35% respectively(P>0.05), The rates in stage Ⅲ-Ⅳand stageⅠ-Ⅱwere 89.66 % and 92.60 % respectively(P>0.05); The positive expression rate of MMP-9 protein in PTC was significantly higher than those of nodular goiter and normal thyroid tissue(P<0.05). and the rates in PTC with lymph node metastasis and without were 92.31 % and 79.07 % respectively(P>0.05),and the rates in stage Ⅲ-Ⅳand stageⅠ-Ⅱwere 89.66 % and 74.07 % respectively(P>0.05). Their expressions of both TSHR and MMP-9 were not related to sex,age and tumor size of the patient(P>0.05). [Conclusion] Over-expression of TSHR might be related to the development of PTC and become a early marker of the thyroid carcinoma. The over-expression of MMP-9 could be related to invasion and metastasis.