CDK2、CDK4基因与自体移植静脉内膜增殖的关系

Expressions of CDK2 and CDK4 in intimal cell proliferation in autologous vein grafts

关键词:  细胞周期蛋白依赖激酶-2；细胞周期蛋白依赖激酶-4；自体静脉移植；细胞周期  内膜增殖

Key words:  CDK2  CDK4  cell cycle  intima hyperplasia  autologous vein graft

摘要:

[目的]了解CDK2、CDK4在大鼠移植血管的表达及对平滑肌细胞增殖的影响。[方法]Wistar大鼠50只，随机分为5组，建立自体静脉移植模型，分别于术后1、2、3、7及14 d取组织形态学观察，并用免疫组织化学和RT-PCR方法检测血管移植后不同时期CDK2、CDK4的表达情况，取正常静脉为对照组。[结果]移植后7 d，内膜厚度与管壁厚度接近高峰，与对照组及移植后1、2、3 d比较，差异有显著性意义（P<0.05）。免疫组织化学显示，移植静脉CDK2、CDK4阳性细胞在移植后2 d明显增加，7 d达到高峰，与移植后1 d比较，差异有显著性意义（P<0.05）。RT-PCR检测结果显示，CDK2、CDK4基因mRNA表达产量7～14 d达到高峰，与移植后1、2、3 d比较，差异有显著性意义（P<0.05）。[结论] CDK2、CDK4蛋白和mRNA表达在移植静脉早期开始增加，在7～14 d达到高峰。在移植静脉内膜增生过程中CDK2、CDK4表达可能起着一定的作用。

Abstract:

[Objecttive]To observe CDK2, CDK4 expressions of proliferative intima in autologous grafted vein of rat. [Methods] Rat autologous vein graft model was established. Fifty rats were divided into five groups randomly, and the graft veins were respectively collectived at the1st, 2nd, 3rd, 7th, and14th day after the operation. Then the protein expressions of CDK2 and CDK4 were detected by immunohistochemistry and the expressions of their mRNA were detected by RT-PCR in intima of the graft vein. Normal veins were used for control. [Results]At 7d after operation, the intima proliferation of autologous grafted vein reached nearly to the high point, and it was more obvious than those of control group and groups at1 d, 2 d and 3 d after operation(P<0.05) . The numbers of CDK2 and CDK4 positive cells in graft vein increased from 2 d after operation and reached to the peak at 7 d after operation. The expressions of CDK2, CDK4 mRNA reached to the peak during 7 d to14 d after operation. [Conclusions] The expressions of CDK2 and CDK4 increased from early phrase after vein graft, and reached the peak during 7 d to14 d after operation. The CDK2 and CDK4 played a role in intima proliferation of autologous graft vein.