引用本文: | 郝立宏,杨晓燕,宫琳琳,赵瑾瑶,宋 阳,杜 莎,王 兰,刘 鹭,熊 海.肺癌细胞株E-cadherin的表达状态及其与启动子甲基化的关系[J].大连医科大学学报,2011,33(4):325-329. |
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肺癌细胞株E-cadherin的表达状态及其与启动子甲基化的关系 |
郝立宏,杨晓燕,宫琳琳,赵瑾瑶,宋 阳,杜 莎,王 兰,刘 鹭,熊 海
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1.大连医科大学 蛋白质组学实验室,辽宁 大连 116044;2.大连医科大学 附属第二医院 肿瘤科,辽宁 大连 116027;3.大连医科大学 附属第一医院 胸外科,辽宁 大连 116001
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摘要: |
[目的]研究肺癌细胞株上皮钙粘蛋白(E-cadherin)的表达与基因启动子甲基化状态的关系。[方法]采用免疫细胞化学法检测肺癌细胞株NCI-H460、SPCA和 A549的E-cadherin蛋白表达;半巢式甲基化特异性PCR法(MSP)和微流控芯片技术检测E-cadherin基因启动子甲基化状态。[结果]E-cadherin蛋白在NCI-H460、SPCA和A549肺癌株均呈弱表达,以A549细胞株表达最低;MSP法和微流控芯片技术在三种细胞株中均检测到E-cadherin基因启动子的甲基化,以A549细胞株启动子甲基化程度最高,二者结果相符。[结论]E-cadherin基因启动子甲基化可能是肺癌细胞株NCI-H460、SPCA和A549出现E-cadherin蛋白表达下调的原因。 |
关键词: 上皮钙粘蛋白 启动子甲基化 MSP 微流控芯片技术 |
DOI:10.11724/jdmu.2011.04.04 |
分类号:R73-3 |
基金项目: |
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Protein expression and promoter methylation status of E-cadherin in lung cancer cell lines |
HAO Li-hong1, YANG Xiao-yan1,2,3, GONG Lin-lin1, ZHAO Jin-yao1, SONG Yang1, DU Sha1, WANG Lan1, LIU Lu1, XIONG Hai1,4
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1.Department of Histology and Embryology,Dalian Medical University,Dalian 116044,China;2.Department of Oncology,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China;3.;4.Department of Thoracic Surgery,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,China
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Abstract: |
[Objective]To investigate the relationship between the promoter methylation status and protein expression of E-cadherin in lung cancer cell lines.[Methods]The protein expressions of
E-cadherin in lung cancer cell lines NCI-H460,SPCA and A549 were examined by immunocytochemistry.The promoter methylation status of E-cadherin was detected by methylation-specific PCR (MSP) and microfluidics.[Results]The weak expressions of E-cadherin in NCI-H460,SPCA and A549 were detected,the weakest in A549.The promoter methylation of E-cadherin occurred in all three cell lines and the highest percentage of methylation in A549,which was in agreement with the protein expression.[Conclusions]The promoter methylation can be a cause of E-cadherin its down-regulation in lung cancer cell lines. |
Key words: E-cadherin methylation MSP microfluidics |