神经生长因子对大鼠脑缺血再灌注后Fas-L蛋白和p-PKB表达的影响

Effect of nerve growth factor on expression of p-PKB and Fas-L gene protein after reperfusion in post cerebral ischemia rats

关键词:  局灶性脑缺血/再灌注  神经生长因子  Fas-L  p-PKB

Key words:  focal cerebral ischemia-reperfusion  nerve growth factor  Fas-L  p-PKB

摘要:

［目的］探讨神经生长因子（NGF）对大鼠脑缺血再灌注后Fas-L和p-PKB表达的影响，为临床应用NGF治疗缺血性脑血管病提供依据。［方法］健康成年SD大鼠55只，随机分为假手术组（n=5）；对照组（n=30），按缺血2 h再灌注2 h、6 h、12 h、24 h、48 h、72 h再分为6组，每组5只；NGF组（第8f和8p组，分别为缺血2 h再灌注6 h和再灌注72 h注射NGF，每组5只）和生理盐水组（第9f和9p组，分别为缺血2 h再灌注6 h和再灌注72 h注射生理盐水，每组5只）。制备MCAO模型。观察各组动物神经功能缺损评分和脑内Fas-L和p-PKB表达。［结果］NGF组与生理盐水组比较，神经功能缺损程度（1.400±0.6253，2.300±0.3650），（2.200±0.6903，3.100±0.0038）均明显减轻,差异有显著性意义（P<0.01）。与缺血再灌注组相比，NGF干预组Fas-L蛋白表达 （18.6600±12.2584）减少，p-PKB表达（66.6600±12.3353）增多，差异有显著性意义（P<0.01）。［结论］肌注NGF对脑缺血再灌注有明显的保护作用。

Abstract:

［Objective］To observe the effect of nerve growth factor on expression of p-PKB and Fas-L gene protein after reperfusion in post cerebral ischemia rats,to provide the theoretical basis for clinical therapy of ischemia cerebrovascular disease. ［Methods］Fifty-five adult SD rats were randomly divided into eleven groups with five in each,including sham group,6 control groups which according to ischemia time were divided into 2 h,6 h,12 h,24 h,48 h and 72 h ones,NGF groups including 8f one,NGF injected after 6 h reperfusing,and 8p group,NGF injected after 72 h reperfusing,and saline groups including 9f,saline injected after 6 h reperfusing and 9p group,saline injected after 72 h reperfusing. MCAO model was set up. Zea long 5 mark system standard grades was observed in each group of rats for nerve function appraisal and the expression of Fas-L and p-PKB protein was examined.［Results］The nerve function grade,compared with normal saline group (2.200±0.6903,3.100±0.0038),disfunction of NGF group (1.400±0.6253,2.300±0.3650) were obviously alleviated (P<0.01);compared with ischemia and reperfusion rats,NGF given in the early stage of ischemia could increase the expression of p-PKB protein(66.6600±12.3353) and reduce the expression of Fas-L protein (18.6600±12.2584,P<0.01). ［Conclusion］Injecting NGF has obviously protective function in the ischemia- reperfusion rats.