大承气汤对重症胰腺炎SIRS大鼠腹腔巨噬细胞sCD14作用的实验研究

Study of function sCD14 of Da Cheng Qi Tang SIRS in severe acute pancreatitis in rat peritoneal macrophages

关键词:  大承气汤  重症急性胰腺炎  SIRS  巨噬细胞  sCD14

Key words:  Da Cheng Qi Tang  severe acute pancreatitis  SIRS  macrophage  sCD14

摘要:

［目的］研究大承气汤对重症胰腺炎SIRS大鼠腹腔巨噬细胞sCD14的作用机制。［方法］建立重症急性胰腺炎（SAP）全身炎症反应综合征（SIRS）大鼠模型。分为5个实验组：正常组、SIRS模型组、中药组、西药组、中西结合组。用酶联免疫分析（ELISA）技术检测不同处理组中sCD14的浓度。 ［结果］检测SAP大鼠腹腔MΦsCD14的浓度发现：中西医结合治疗组明显低于SIRS组（31.83±29.51）vs（272.82±40.68），P<0.05；中西医结合治疗组低于中药组和西药组，分别为（31.83±29.51）vs（88.97±54.8），P<0.05和（31.83±29.51）vs（83.45±35.21），P<0.05；中西医结合治疗组与正常组比较差异无显著性意义（31.83±29.51）vs（31.30±24.30），P>0.05。［结论］大承气汤治疗重症急性胰腺炎/全身炎症反应综合征时对腹腔MΦsCD14有明显的抑制作用。

Abstract:

［Objective］To study the function of applied Da Cheng Qi Tang to SIRS in severe acute pancreatitis in SAP/SIRS rat peritoneal macrophage sCD14.［Methods］Severe acute pancreatitis SIRS rat model was set up.Five groups included control,SIRS,traditional medicine,western medicine,integrated traditional and western medicine groups.Concentration of sCD14 was detected by ELISA in all of the groups.［Results］PMΦs in integrated traditional and western medicine groups were obviously lower than that in SIRS group (31.83±29.51) vs(272.82±40.68),P<0.05,and it in integrated traditional and western medicine group was obviously lower than that in traditional medicine and western medicine groups respectively (31.83±29.51) vs (88.97±54.80),P<0.05;(31.83±29.51) vs (83.45±35.21),P<0.05.There was no difference between integrated traditional and western medicine group and control group (31.83±29.51) vs (31.30±24.30),P>0.05.［Conclusion］Da Cheng Qi Tang treatment has inhibiting effect on PMΦ sCD14 in SAP/ SIRS.