p38MAPK在低能ESW和间歇rhPTH1-34促进ROB成骨的信号转导中的作用

Role of p38MAPK in signal transduction of low density ESW and low dose intermittent rhPTH1-34′s action on bone formation of cultured rat osteoblasts

关键词:  成骨细胞  体外冲击波  rhPTH1-34  p38MAPK

Key words:  osteoblast  extracorporeal shock wave (ESW)  rhPTH1-34  p38MAPK

摘要:

［目的］探讨低能体外冲击波（ESW）和间歇人重组甲状旁腺素（rhPTH1-34）刺激引起的体外培养大鼠成骨细胞（ROB）成骨的细胞内信号转导中p38MAPK的作用。［方法］分别用有效的低能ESW刺激和间歇rhPTH1-34作用于ROB，并设立加入p38MAPK抑制剂SB203580组，来观察ROB增殖、成骨指标及Western Blot检测的p38MAPK磷酸化激活变化。［结果］p38MAPK抑制剂SB203580能明显抑制120次0.18 mJ/mm2 ESW刺激引起的ROB细胞增殖和成骨作用（P<0.05）；但不能明显抑制10-11 mol/L间歇rhPTH1-34刺激引起的体外培养ROB细胞增殖及成骨作用（P>0.05）。ESW应力刺激可促进p-p38MAPK表达，但间歇rhPTH1-34不能促进p-p38MAPK表达。［结论］适当的ESW应力刺激可通过激活p38MAPK促进体外培养ROB增殖和成骨；但p38MAPK并不参与10-11 mol/L间歇rhPTH1-34刺激引起的ROB细胞增殖和成骨的细胞信号转导。

Abstract:

［Objective］To investigate the role of p38MAPK in the signal path way of low density ESW and low dose intermittent rhPTH1-34 stimulation′s action on rat osteoblasts.［Methods］After stimulations of 0.18 mJ/mm2 ESW and 10-11 mol/L intermittent rhPTH1-34,the specific inhibitor of p38MAPK was added for finding the role of p38MAPK in the intracellular signal path way.The rat osteoblasts were collected,and cultured was detected,proliferation of these cells was observed by MTT and bone formation by measuring ALP activity.The expression of p-p38MAPK was detected by Western blot.［Results］Enhaning effect on bone formation by ESW can be significantly inhibited by SB203580,a inhibitor of p38MAPK (P<0.05).But the enhancement of ROBs’ bone formation by intermittent rhPTH1-34 can not be inhibited by SB203580.Suitable ESW stimulation can improve the expression of p38MAPK,but intermittent rhPTH1-34 stimulation can not.［Conclusions］Suitable ESW stimulation can enhance bone formation of ROB by activation of p38MAPK.The enhancement of ROBs’ bone formation by intermittent rhPTH1-34 stimulation dosen’t depend on activation of p38MAPK.