| 摘要: |
| ［目的］采用实时荧光定量PCR的方法检测乙型肝炎患者外周血单个核细胞中HBV cccDNA 的含量，并探讨其临床意义。［方法］应用实时荧光PCR（RT-PCR）方法对随机选取的乙型肝炎患者100例和1例追踪治疗的乙肝患者的血清HBV DNA 含量和外周血单个核细胞中HBV cccDNA 的含量进行检测；对已确诊的慢性乙肝患者40例，肝硬化患者30例外周血单个核细胞中HBV cccDNA 的含量进行检测。［结果］100例乙型肝炎患者中，HBV DNA含量<5×102 copies／mL有57例，HBV cccDNA 的含量均<5×102 copies／mL；HBV DNA含量>5×102 copies／mL有43例，HBV cccDNA 的含量>5×102 copies／mL有22例；HBV DNA含量>1×103 copies／mL的患者有17例，在这些患者中HBV cccDNA 的含量>5×102 copies／mL者为13例，两者之间比例经χ2 检验，差异无显著性意义，P>0.05；肝硬化患者HBV cccDNA 阳性率（>5×102 copies／mL）为67.7%，远远高于慢性乙肝患者的42.5%和随机乙肝人群的22.0%。各组间患者比例经χ2 检验，差异有显著性意义，P<0.05。［结论］乙型肝炎患者进行外周血单个核细胞中HBV cccDNA的检测，对于抗病毒治疗效果的监测和预后判断都重要的临床意义。 |
| 关键词: HBV cccDNA 单个核细胞 实时荧光PCR |
| DOI：10.11724/jdmu.2010.3.30 |
| 分类号:R466.1 |
| 基金项目: |
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| Detection of HBV cccDNA of peripheral blood mononuclear cells in hepatitis B patients and its clinical significance |
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XIAO Xiao-guang1, LI Yan-lian1, WANG Jing1, QU Shu-xian2
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1.Department of Clinical Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;2.Key Laboratory of Medical Cellular and Molecular Biology, Dalian Medical University, Dalian 116044, China
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| Abstract: |
| ［Objective］To detect HBV cccDNA levels of peripheral blood mononuclear cells in hepatitis B patients with RT-PCR,and discuss its clinical significance.［Methods］Serum and peripheral blood mononuclear cell samples were collected from 100 random patients with HBV infection, 40 patients with clinical diagnosis of chronic hepatitis B, 30 liver cirrhosis patients with HbsAg (+), and 1 acute hepatitis B patient during therapy. HBV cccDNA and HBV DNA levels were tested by RT-PCR.［Results］In the 100 patients with HBV infection, HBV DNA levels <5×102 copies / mL were 57, in which, HBV cccDNA levels were all <5×102 copies/mL; HBV DNA levels>5×102 copies / mL were 43(43%), HBV DNA levels >5×102 copies / mL were 22 (22%). In 43 HBV DNA positive (> 5×102 copies / mL ) samples, HBV DNA >1×105 copies /mL (strong positive ) were 17, HBV cccDNA >5 × 102 copies / mL were 13. There was not significant difference between these groups(P>0.05). On the other hand, positive rate of HBV cccDNA in cirrhosis patients' peripheral blood mononuclear cell samples (> 5×102 copies / mL) was 67.7%, which was much higher than that of chronic hepatitis B patients (42.5%) and hepatitis B random groups (22.0%). There was significant difference between them(P<0.05).［Conclusion］RT-PCR detecting HBV cccDNA of peripheral blood mononuclear cells in hepatitis B patients has important clinical significance for monitoring the curative effect and determining their prognosis. |
| Key words: HBV cccDNA mononuclear cells RT-PCR |
