降低氯化锂-匹罗卡品大鼠癫痫模型死亡率的实验研究

Experimental study on the mortality of reducing the epilepsy rat model induced by LiCl-pilocarpine

关键词:  氯化锂-匹罗卡品  癫痫  水合氯醛  死亡率

Key words:  LiCl-pilocarpine  epilepsy  chloral hydrate  mortality

摘要:

［目的］探讨有效控制氯化锂-匹罗卡品诱发癫痫持续状态，提高癫痫大鼠存活率的最佳条件。［方法］选用SD大鼠30只，腹腔注射氯化锂和匹罗卡品后，诱发大鼠癫痫持续状态1 h后，随机分为A组：只给东莨菪碱；B组：东莨菪碱+安定；C组：东莨菪碱+水合氯醛。比较3组大鼠间癫痫发作的持续状态、癫痫大鼠的死亡率、死亡发生的时间。［结果］A组大鼠癫痫发作的持续状态为16~20 h，死亡率为87.5%；B组大鼠在用药后15 min内可控制癫痫发作的持续状态，死亡率为62.5%；C组大鼠在用药后3~10 min可完全控制大鼠的癫痫发作持续状态，大鼠全部存活。［结论］水合氯醛可有效控制氯化锂-匹罗卡品诱发大鼠的癫痫持续状态，提高癫痫大鼠的存活率。

Abstract:

［Objective］To explore the effective control of persisting epilepsy status induced by LiCl-pilocarpine and the optimal condition of reducing the death rate of these animals.［Methods］Thirty SD rats were injected with LiCl-pilocarpine intraperitoneally to induce their persisting epilepsy status.One hour later,they were randomly divided into group A only administrated with scopolamine,group B with scopolamine and diazepam,and group C with scopolamine and chloral hydrate.The indexes including the rats epilepsy plexy persisting status,mortality,and death time were observed and compared respectively.［Results］The epilepsy plexy persisting status of the rats in group A was lasting for 16 to 20 hours with the death rate of 87.5%.The persisting status of the rats in group B was controlled within 15 minutes with the death rate of 62.5 %.The symptom of the animals in group C was completely controlled within 3 to 10 minutes after administration and all the rats survived.［Conclusion］Chloral hydrate could effectively control the experimental rat s epilepsy persisting status and decrease the their mortality.