低分子肝素对肾小球系膜细胞产生细胞外基质的影响

Effect of low molecular weight heparin on glomerular mesangial cells toproduce of extracellular matrix

关键词:  低分子肝素  系膜细胞  基质

Key words:  fluvastatin  mesangial cell  matrix

摘要:

［目的］了解不同葡萄糖浓度环境下，低分子肝素对人系膜细胞表达纤维连接蛋白(FN)和IV型胶原作用。［方法］培养人肾小球系膜细胞，分为正常葡萄糖组、高葡萄糖组及加低分子肝素组，培养24、48 h收集细胞上清液，用酶联免疫法检测FN和IV型胶原。［结果］高糖组24和48 h FN分别为（1.552±0.546）ng/mL和（2.547±0.968）ng/mL 明显高于正常糖组（0.777±0.076）ng/mL和（1.180±0.091）ng/mL（P<0.01）；IV型胶原分别为（64.577±5.543）ng/mL和（80.140±6.605）ng/mL，明显高于正常糖组（42.468±5.615）ng/mL和（46.368±5.714）ng/mL（P<0.01）。在低分子肝素1 ng/mL 的作用下，高糖组的FN24 h及FN48 h分别降低为（1.070±0.349）ng/mL及（1.918±0.074）ng/mL，IV型胶原24 h及IV型胶原48 h分别降低至（41.993±7.470）ng/mL及（40.490±4.377）ng/mL。低分子肝素提高至10 ng/mL时，高糖组的FN24 h及FN48 h分别降低为（0.658±0.071）ng/mL及（0.280±0.035）ng/mL，IV型胶原24 h及IV型胶原48 h分别降低至（34.717±4.505）ng/mL及（27.902±5.325）ng/mL（P<0.01）。［结论］低分子肝素可抑制高糖刺激的系膜细胞表达FN、Ⅳ型胶原。

Abstract:

［Objective］To observe the effect of glucose concentration,low molecular weight heparin on human mesangial cells to produce FN and collagen Ⅳ.［Methods］Human mesangial cells were cultured.Then all cultured cells were divided into normal glucose group,high glucose group and experimental groups .Human mesangial cells were incubated with different glucose (5 mmol/L or 25 mmol/L) with or without different concentrations of low molecular weight heparin for 48 hours.Type Ⅳcollagen and fibronectin ( FN) levels were assessed by ELISA.［Results］Human glomerular mesangial cells in different glucose expressed FN and type Ⅳ collagen.Low molecular weight heparin decreased the level of cell culture supernatant of FN and type Ⅳ collagen in high glucose.The levels of FN in 25 mmol/L glucose at 24 or 48 hours being (1.552±0.546) ng/mL and (2.547±0.968) ng/mL were significantly higher than those in 5.0 mmol/L glucose being (0.777±0.076) ng/mL and (1.180±0.091) ng/mL (P<0.01).The levels type Ⅳ collagen in 25 mmol/L glucose at 24 or 48 hours being (64.577±5.543) ng/mL and (80.140±6.605) ng/mL were significantly higher than those in 5.0 mmol/L glucose being (42.468±5.615) ng/mL and (46.368±5.714) ng/mL (P<0.01).In the 25 mmol/L glucose group with low molecular weight heparin (1 mg/L),FN24 h and FN48 h decreased to (1.070±0.349) ng/mL and (1.918±0.074) ng/mL respectively,and the type Ⅳ collagen24 h type and Ⅳ collagen48 h decreased to (41.993±7.470) ng/mL and (40.490±4.377)ng/mL respectively. In the 25 mmol/L glucose group with low molecular weight heparin (10 mg/L),FN24 h and FN48 h decreased to (0.658±0.071) ng/mL and (0.280±0.035) ng/mL respectively,and the type Ⅳ collagen24 h or type Ⅳ collagen48 h decreased to (34.717±4.505)ng/mL and (27.902±5.325) ng/mL respectively(P<0.01).［Conclusion］Low molecular weight heparin may be decrease FN and type Ⅳ collagen expressions of cultured mesangial cell by high glucose stimulation.