| 摘要: |
| ［目的］了解耐亚胺培南的铜绿假单胞菌产β-内酰胺酶的分布及分离率。［方法］采用K-B法进行药物敏感试验，筛选耐亚胺培南的铜绿假单胞菌；再进行改良三维实验，对菌株所产β-内酰胺酶进行分析。［结果］临床分离耐亚胺培南的铜绿假单胞菌40株中，单产超广谱β-内酰胺酶（ESBLs）9株（22.5%），单产高产染色体头孢菌素酶（AmpC）2株（5.0%），5株为超超广谱β-内酰胺酶（SSBL）（12.5%），3株产酶量过高或其他原因（7.5%），21株无酶活性（52.5%）。［结论］产β-内酰胺酶是耐亚胺培南铜绿假单胞菌的主要耐药机制之一。 |
| 关键词: 铜绿假单胞菌 β-内酰胺酶 三维实验 耐药 亚胺培南 |
| DOI：10.11724/jdmu.2009.05.23 |
| 分类号:R466.1 |
| 基金项目: |
|
| Three-dimensional method applied to detect beta-lactamases in imipenem-resistant pseudomonas aeruginosa |
|
ZHANG Feng-hua1, YUAN Hong1, XIAO Xiao-guang1, WANG Jing1, CHEN Jie1, JIA Yu-jie2
|
|
1.Department of Medical Microbiology Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;2.Department of Aathophysiology, Dalian Medical University, Dalian 116044, China
|
| Abstract: |
| ［Objective］ To analyze beta-lactamase in imipenem-resistant pseudomonas aeruginosa. ［Methods］ At first, the strains of imipenem-resistant P. Aeruginosa were selected with K-B susceptibility method. Then the three-dimensional method was taken to differentiate and analyze the various beta-lactamases. ［Results］ The enzymes produced by 40 strains imipenem-resistant P. Aeruginosa included extended spectrum beta-lactamases (ESBLs) (22.5%). High level AmpC beta-lactamases (5.0%), SSBL (12.5%), enzyme production were too high or other reasons (7.5%) and no activity (52.5%). ［Conclusion］ Production of beta-lactamases is one of the important reasons of imipenem -resistant P. aeruginosa. |
| Key words: pseudomonas aeruginosa beta-lactamases three-dimensional method resistance imipenem |
