对肝硬化腹水进行净化并超滤浓缩的实验研究

Study on endotoxin and bilirubin removal by CA/PEI affinity membrane and protein ultrafiltration by polysulfone hollow fiber membrane in ascites of cirrhosis

关键词:  CA/PEI亲和膜  中空纤维膜  内毒素  胆红素  浓缩

Key words:  CA/PEI affinity membrane  hollow fiber membrane  endotoxin  bilirubin  concentration

摘要:

［目的］净化和优化肝硬化腹水使其更有利于浓缩回输治疗。［方法］采用CA/PEI亲和膜去除腹水胆红素、内毒素，并采用聚砜中空纤维膜超滤浓缩腹水，用内毒素测定仪、生化自动分析仪和免疫分析仪测定过膜前后各项指标。［结果］CA/PEI亲和膜吸咐前后腹水中的胆红素、内毒素均明显下降（P<0.01）；胆红素吸附前（51.77±11.76）μmol/L；吸附后（21.18±6.83）μmol/L；内毒素吸附前（3.777±0.233）EU/mL，吸附后（0.341±0.322）EU/mL。白蛋白、离子、免疫球蛋白均无明显变化（P>0.05）。腹水超滤浓缩5.5~6.5倍，蛋白质浓缩4.7~6.5倍，免疫球蛋白浓缩3倍，但离子无变化。［结论］用CA/PEI亲和膜可去除胆红素、内毒素，用聚砜中空纤维膜超滤浓缩能使腹水得到净化和蛋白浓缩。

Abstract:

［Objective］ Purification and optimization of cirrhotic ascites is more helpful to reinfusion and treatment of ascites. ［Methods］ Endotoxin and bilirubin removal by CA/PEI affinity membrane and protein ultrafiltration by polysulfone hollow fiber membrane in ascites of cirrhosis were studied. The indexes of pre and post trans-membrane by endotoxin detector, biochemistry analyzer and immunity analyzer were measured. ［Results］ The endotoxin and bilirubin concentration in ascites were reduced with adsorption by CA/PEI affinity membrane (P<0.01). There were unchanged in albumin, ion and immunoglobulin (P>0.05). Before adsorptioned bilirubin is (51.77±11.76)μmol/L,after which is (21.18±6.83)μmol/L. Before adsorptioned endotoxin is (3.777±0.233)EU/mL,after which is(0.341±0.322)EU/mL.Ascites were concentrated 5.5 to 6.5 times by ultrafiltration, while the protein in ascites was concentrated 4.7 to 6.5 times and immunoglobulin was concentrated more than 3 times and ions were no change. ［Conclusion］ Ascites of purification and protein concentration may be obtained by the way of removal endotoxin and bilirubin with CA/PEI affinity membrane and ultrafiltration and concentration with polysulfone hollow fiber membrane.