石蜡半薄切片制作透射电镜的方法

Sample preparation methods of TEM using semi-thin paraffin sections

关键词:  石蜡半薄切片  电镜样品制作  透射电镜

Key words:  semi-thin paraffin sections  electron microscopy sample production  transmission electron microscopy

摘要:

［目的］介绍石蜡半薄切片转制透射电镜的制样方法。［方法］对石蜡半薄切片进行脱蜡、水化、锇酸固定、缓冲液漂洗、梯度酒精脱水、环氧树脂包埋、超薄切片机切片、透射电镜下观察。［结果］组织细胞的超微结构保存完好，足以满足病理诊断之用。［结论］用石蜡半薄切片转制电镜比用石蜡组织块转制电镜方法简单，快速易行。

Abstract:

［Objective］ To introduce sample preparation methods of TEM using semi-thin paraffin sections. ［Methods］ The semi-thin paraffin section was processed with dewaxing, hydration, osmium tetroxide fixed, buffer rinsing, gradient alcohol dehydration, epoxy resin embedded and ultra-thin slice. Then the sections were observed under the Transmission Electron Microscopy. ［Results］ The ultra-structure of histo-cyte remained well. It was sufficient to meet the requirement of pathological diagnosis. ［Conclusion］ Compared with paraffin tissue samples, using semi-thin paraffin sections are more efficiency.