邻苯二甲酸酯对小鼠雄性生殖系统的毒性作用

Preconception injuries of male mice induced by diethylhexyl phthalate

关键词:  邻苯二甲酸（2-乙基已基）酯（DEHP）  雄性  生殖毒性  氧化应激

Key words:  Diethylhexyl phthalate  male  preconception injuries  oxidative stress

摘要:

［目的］探讨邻苯二甲酸(2-乙基已基)（Diethylhexyl phthalate，DEHP）对小鼠雄性生殖系统的毒性作用机制。［方法］昆明种雄性小鼠（20±2）g，40只，随机分为4组，每组10只。经食饵自然给食染毒，连续4周后处死。观察小鼠体重及睾丸变化，测定不同剂量DEHP染毒小鼠的血液、睾丸中谷胱甘肽过氧化物酶（GPX）活性、一氧化氮（NO）和过氧化氢（H₂O₂）含量。［结果］DEHP染毒后，随着染毒剂量增加，小鼠体重逐渐减少（P<0.01），高剂量组睾丸脏器系数显著下降（P<0.01）。光镜下可见高剂量组睾丸组织有明显损伤。与对照组相比，DEHP中、高剂量暴露组小鼠血液及睾丸中GPX活性降低（P<0.01）；H₂O₂ 含量增加（P<0.05，P<0.01）；睾丸中NO含量降低，尤其是高剂量组与对照组差别明显（P<0.05，P<0.01）。［结论］DEHP对小鼠雄性生殖系统具有明显的毒性作用，其作用机制可能与氧化应激反应和NO含量降低有关。

Abstract:

［Objective］ To detect the mechanism of male preconception injuries induced by diethylhexyl phthalate (DEHP) in mice.［Methods］ Forty of Kumming mice (20±2)g were divided into 4 groups randomly,every group including 10 mice. The animals were exposed to DEHP by food for 4 weeks.At the end of the fourth week,they were terminated.Then calculated the weight and coeffcient of testis/body. GPX、NO、H₂O₂ of blood and testis were examined.［Results］ With the increase of DEHP ,the weight of mice and the coeffcient of testis/body with the high dose group decreased significantly (P<0.01). There were evident testes tissue damage in high dose group with examination by light microscope. Comparing with the control group,GPX activity in the blood and testes decreased dramatically (P<0.01), while H₂O₂ content increased significantly (P<0.05,P<0.01); in modprote and high dose qroups NO content decreased dramatically in testes,especially in high dose of DEHP (P<0.05).［Conclusion］ DEHP has significant male preconception injuries effect to mice. The possible mechanisms are oxidative stress injuries and NO content decrease.