非小细胞肺癌体外化疗药物敏感性与GRP94表达的相关性

Relationship between chemosensitivity and expression of GRP94 in non-small cell lung cancer in vitro

关键词:  NSCLC  GRP78  体外药敏实验  耐药

Key words:  NSCLC  GRP94  MTT  chemosensitivity in vitro  resistance

摘要:

［目的］ 探讨非小细胞肺癌（NSCLC）对8种化疗药物的体外敏感性与GRP94表达的相关性。［方法］用四噻唑蓝（MTT）法检测52例手术切除新鲜NSCLC组织8种化疗药物体外敏感性；免疫组织化学方法检测肺癌组织GRP94表达。采用Spearman法进行相关性分析。［结果］ NSCLC52例对紫杉醇（PTX）、阿霉素（ADM）、卡铂（CBP）、拓扑替康（TPT）、长春瑞滨（NVB）、长春新碱（VCR）、顺铂（DDP）和鬼臼乙叉苷（VP-16） 8种化疗药物的耐药率分别为42.31%、57.69%、63.46%、65.38%、67.31%、73.08 %、78.85%和90.38%。其中，14例表现为完全耐药；且其高表达与肺癌细胞对VP-16的耐药明显相关（P<0.05）。［结论］ 检测GRP94表达对推测NSCLC对VP-16的耐药性具有参考意义。

Abstract:

［Objective］ The aim of the research was to study the relationship between chemotherapy resistance and the expression of GRP94 in NSCLC. ［Method］ Drug sensitivity test was used to detect the resistance to 8 chemotherapy drugs in 52 NSCLC fresh surgical samples by (methylthiazoletrazolium) MTT; the expression of GRP94 was detected by immunohistochemistry. Spearman correlation assay was used to investigate the correlation between GRP94 expression and chemotherapy resistance. ［Results］ The resistance rates to paclitaxel(PTX), adriamycin(ADM),carboplatin (CBP),topotecan(TPT),navelbine(NVB),vincristine(VCR)、cisplatin(DDP) and etoposide (VP-16) of the 52 samples were: 42.31%, 57.69%, 63.46%, 65.38%, 67.31%, 73.08 %, 78.85% and 90.38%, respectively. The complete resistance to the total 8 chemotherapy drugs was found in 14 cases. There was a significant over-expression of GPR94 in NSCLC tissues. Spearman correlation assay shown that there was correlation between the chemotherapeutics resistance to VP-16 and the expression of GRP94 in NSCLC(P<0.05).［Conclusions］ The detection of GRP94 may be helpful to the resistance to VP-16 in NSCLC.