茶多酚对小鼠脑缺血再灌注损伤的保护作用

Protective effects of Tea ployphenol injection on cerebral ischemia reperfusion injury in mice

关键词:  茶多酚  脑缺血再灌注  脑水肿  超微结构

Key words:  Tea Ployphenol  cerebral ischemia reperfusion  cerebral edema  urtralstructure

摘要:

［目的］研究茶多酚静脉给药对脑缺血再灌注损伤保护作用并初步探讨其量效、时效关系。［方法］采用双侧颈总动脉结扎/复灌制备小鼠不完全性全脑缺血再灌注损伤模型，茶多酚不同剂量（50、100、200 mg/kg）和不同时间（缺血0 h、再灌即刻、再灌30 min）1次静脉注射给药，观察其对脑缺血再灌注损伤时脑含水量、电镜下超微组织结构变化的影响。［结果］茶多酚50、100 mg/kg、缺血0 h、再灌即刻、再灌注30 min给药组小鼠脑组织含水量（79.59±0.45）%，（79.18±0.45）%，（79.18±0.45）%，（78.95±0.79）%，（79.22±0.89）%与模型组（80.21±0.40）%比较明显降低（P<0.01）；电镜显示可减轻血脑屏障的损伤。［结论］茶多酚静脉注射给药对小鼠脑缺血再灌注损伤具有保护作用，单次用药以再灌注即刻给药作用显著。

Abstract:

［Objective］ To observe the neuroprotective effect and therapeutic window of tea ployphenol (TP) injection on cerebral ischemia reperfusion injury in mice.［Methods］ The cerebral ischemia reperfusion model in mice was made by bilateral ligation of common carotid arteries for 10 minutes and reperfusion for 10 minutes, repeating for three times.Mices were arranged in 9 groups which were sham operation group, ischemia control group, nimodipine group, 3 TP groups treated with different levels of dose (50,100,200 mg/kg ,i.v. 0h before the first ligation), 3 TP groups treated with different administrated time (0 h before first ligation and 0 h,30 min after last reperfusion, i.v. 100 mg/kg).The content of water in the cerebric tissues were measured and the changes of urtralstructure of BBB was observed under electron microscope(EM). ［Results］①The content of brain water in TP 50 mg/kg,100 mg/kg, 0 h before first ligation and 0 h,30 min after last reperfusion groups (79.59±0.45)%, (79.18±0.45)%, (79.18±0.45)%, (78.95±0.79)%, (79.22±0.89)% were less than that in ischemia control group(80.21±0.40%), P<0.01.② The observation under electron microscope demonstrated that the severity of ischemic damage in TP groups was lighter than that in ischemic control group.［Conclusions］The result indicated the neuroprotective effect of TP injection on cerebral ischemia reperfusion injury in mice.