骨髓基质干细胞不同移植途径对脑缺血治疗的影响

Therapeutic effect of different administration channel of bone marrow stromal cells for treating transient focal cerebral ischemia in rats

关键词:  干细胞移植  脑缺血  Bcl-2  Bax  血管内皮生长因子

Key words:  stem cells transplantation  cerebral ischemia  Bcl-2  Bax  VEGF

摘要:

［目的］探讨骨髓基质干细胞（bone marrow stromal cells，MSCs）不同途径移植后在急性局灶性脑缺血疾病治疗中的作用及其机制。 ［方法］ 利用线拴法制作SD大鼠大脑中动脉缺血模型（MCAO）（n=80），随机均分为：缺血自然恢复组（A组），缺血+磷酸盐缓冲液（PBS）对照组（B组），缺血+颈内动脉MSCs组（C组）和缺血+尾静脉MSCs组（D组），分别通过颈内动脉、尾静脉移植相同数量MSCs。再灌注24 h后，分别观察神经行为学评分，脑梗死体积，尼氏小体平均灰度，凋亡相关基因Bcl-2，Bax及血管内皮生长因子（vascular endothelial growth factor，VEGF）的表达。［结果］通过对神经行为学评分，脑梗死体积和病理形态学（尼氏小体、Bcl-2、Bax免疫组化以及VEGF的表达）分析，C组最终的各种指标分析比较均要优于其他3组，之间的差异有显著性意义（P<0.05）。［结论］ 颈内动脉移植MSCs治疗缺血性脑损伤的疗效明显优于尾静脉移植相同数量的MSCs。

Abstract:

［Objective］ To investigate the effect and mechanism of bone marrow stromal cells (MSCs) transplantation for treating cerebral ischemia in rats. ［Methods］ Rat middle cerebral artery occlusion (MCAO) model was established. A total of 80 SD rats were divided into four group, MSCs ( 2×106 ) labeled was transferred into every group, through external carotid artery 24 h after stroke. Behavioral tests were performed before and after MCAO. Immunohistochemistry was used to identify MSCs and expression of neurotrophic factors. ［Results］ The transferred MSCs survived, migrated and producing neurotrophic factors, such as VEGF after the transplantation. Compared with other three groups, the function of nervous system was significantly improved ( P<0.05 ). And, the therapeutic effect of the different administration channel, C group was better than the D group. ［Conclusions］ The therapy of MSCs transferring into rat brain after cerebral ischemia is effective. The neurotrophic factors produced by MSCs may explain the protective mechanism.