乌苏里藜芦生物碱对血管紧张素Ⅱ诱导的乳鼠心肌细胞肥大的影响

Effects of Veratrum nigrum L. Var.ussurience Nakai alkaloids on angiotensin Ⅱ- induced hypertrophy of cardiomyocytes from neonatal rat

关键词:  血管紧张素Ⅱ  乌苏里藜芦生物碱  心肌细胞肥大  钙调神经磷酸酶

Key words:  angiotensin Ⅱ  Veratrum nigrum L. Var.ussurience Nakai alkaloids  cardiomyocyte hypertrophy  calcineurin

摘要:

［目的］在原代培养的大鼠乳鼠心肌细胞上,观察乌苏里藜芦生物碱（Veratrum nigrum L. Var.ussurience Nakai alkaloids，VnA）对血管紧张素Ⅱ（angiotensin Ⅱ，Ang Ⅱ）诱导的大鼠乳鼠的心肌细胞肥大的影响。［方法］通过BCA法测定心肌细胞蛋白含量和显微测微器测定心肌细胞直径，免疫荧光法分析心肌细胞内钙调神经磷酸酶（calcineurin，CaN）的表达变化。［结果］在一定剂量范围内VnA能抑制心肌细胞蛋白含量的增加（P＜0.01）和细胞体积的增加（P＜0.01），细胞内CaN的表达减少。［结论］VnA抑制Ang Ⅱ诱导的乳鼠心肌细胞肥大可能与其抑制细胞内CaN表达有关。

Abstract:

［Objective］To investigate the effect of Veratrum nigrum L. Var.ussurience Nakai alkaloids(VnA) on the hypertrophy induced by using angiotensin Ⅱ(Ang Ⅱ) in the primary culture of neonatal rat cardiomyocytes.［Methods］The cell protein content and the cell diameter were measured using BCA method and the micrometer, and the expressions of calcineurin(CaN) in the cardiomyocytes were determined using immunofluorescence analysis.［Results］VnA inhibited the increase of protein content, the cell diameter in the AngⅡ-induced neonatal rat cellular hypertrophy in a dose-dependent manner. The increase of expression of CaN was also inhibited by using VnA.［Conclusion］The results suggest that the mechanism for VnA inhibiting the hypertrophy of cardiomyocytes induced by Ang Ⅱ be related to the inhibition of CaN expression.