肥胖SD大鼠肝脏组织中SOCS3 mRNA的表达

Study on expression of liver SOCS3 mRNA in obese rat fed with high-fat diet

关键词:  饮食诱导的肥胖（DIO）  leptin  leptin抵抗  SOCS3

Key words:  diet-induced-obesity  leptin  obesity leptin-resistance  SOCS3

摘要:

［目的］初步探讨肥胖SD大鼠肝脏组织中细胞因子信号传导抑制因子3（suppressor of cytokine signaling 3,SOCS3) mRNA的含量与高瘦素（leptin）血症的关系。［方法］采用高脂饲料喂养以诱导的肥胖（DIO）SD大鼠模型，应用放射免疫分析法检测血清leptin水平，应用RT-PCR半定量法检测肝脏组织中SOCS3 mRNA的含量，并进行相关分析。［结果］DIO组大鼠存在高leptin血症，其肝脏组织中SOCS3 mRNA的含量0.9392± 0.0081明显高于正常饮食组大鼠0.3460±0.0743，两组间差异有显著性意义（P＜0.01），而且肝脏组织中SOCS3 mRNA含量与大鼠的肥胖程度及血清leptin水平呈显著正相关，P<0.01。［结论］肝脏组织中SOCS3可能是DIO大鼠的leptin抵抗的机制之一。

Abstract:

［Objective］To investigate the relationship between the expression of liver (suppressor of cytokine signaling 3,SOCS3) mRNA and serum leptin concentration.［Methods］To duplicate the obese rat model by feeding with high-fat diet (diet-induced-obesity rats, DIO group), and utilize the radioimmunity to deteet the serum leptin concentration, semi-quantitative RT-PCR to measure SOCS3 mRNA value in rat liver tisrue and then analyze the relationships between them.［Results］In DIO group, the concentration of serum leptin 0.9392±0.0081 was higher than that in control group with normal diet 0.3460±0.0743, and the expression of SOCS3 mRNA in liver tisscce of DIO group is also higher than that of cowtrol with significant difference (P<0.01). The expression of SOCS3 mRNA in liver tissve has a significant positive relation to the level of serum leptin.［Conclusion］High expression of SOCS3 mRNA in liver may be an important mechanism for obesity leptin-resistance of DIO rat.