| 摘要: |
| ［目的］了解大连地区耐环丙沙星的大肠埃希菌中qnrA基因的流行情况并研究其耐药机制。［方法］收集临床分离的耐环丙沙星的大肠埃希菌213株，通过PCR法扩增qnrA基因，对qnrA基因阳性株，KB纸片法检测抗菌药的体外抗菌活性，琼脂稀释法测MIC，并进行质粒结合试验。［结果］213株菌中查到2株qnrA阳性菌株，测序结果与Genbank中qnrA基因比对吻合率达100%。其中一株对多种抗菌药耐药，另一株则比较敏感。［结论］大连地区检测到了qnrA基因阳性的菌株，耐药机制复杂，应引起临床重视。 |
| 关键词: qnrA基因 环丙沙星 大肠埃希菌 |
| DOI：10.11724/jdmu.2008.03.24 |
| 分类号:R446 |
| 基金项目: |
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| Detection of qnrA gene in Escherichia coli |
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ZHANG Peng, NIE Da-ping
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Department of Clinical Laboratory,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China
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| Abstract: |
| ［Objective］To investigate the prevalence of qnrA gene in Escherichia coli in Dalian,and study it’s mechanism of the resistance.［Methods］A total of 213 clinical isolates of quinolone resistance in Escherichia coli.The qnrA genes were amplified by PCR.Kirby-Bauer disk diffusion method was used to test the in vitro susceptibility of the isolates to antimicrobial agents.MIC values were determind for qnrA gene positive strains.Plasmid conjugation experments were done in order to investigate the qnrA genes can transfer or not.［Results］The qnrA genes were identified in 2 clinical isolates and the qnrA gene sequence matched that of GenBank with 100%.One was resistant to many antimicrobial agents,another was not.The plasmid conjugation tests were carried out in the both strains.［Conclusions］The qnrA genes have been existing in DaLian.The serious situation of complex resistance mechanism should be highly concerned in clinics. |
| Key words: qnrA gene Ciprofloxacin Escherichia coli |
