伊立替康及联合5-氟尿嘧啶、顺铂对人肝癌细胞株的细胞毒作用实验研究

Experimental study of cytotoxicity of CPT-11 and combinated with 5-FU and DDP on human hepatocellular carcinoma strain

关键词:  伊立替康  5-氟尿嘧啶  顺铂  原发性肝癌  HepG2细胞株

Key words:  hepatocellular carcinoma  Irinotecan  HepG2 cell strain  5-FU  DDP

摘要:

［目的］探讨伊立替康(CPT-11)及联合5-氟尿嘧啶(5-FU)、顺铂(DDP)对人肝癌细胞株的细胞毒性作用。［方法］利用MTT比色法检测CPT-11，5-FU，DDP 3种药物对人肝癌HepG2细胞株的抑制率，进一步测定3种药物单药及联合用药对细胞生长抑制的情况并进行比较。［结果］不同浓度CPT-11，5-FU，DDP作用细胞后，细胞生长受到不同程度的抑制，这一抑制作用随药物浓度的升高而增强（P<0.01）, 单药对肝癌细胞株抑制作用最强的为CPT-11，其次为DDP，5-FU（三药作用于相同起始浓度的肝癌细胞株48、72、96、120 h后细胞数量分别为 4.70±0.25、12.71±0.23、20.30±1.59、24.20±2.31；6.50±0.81、16.25±0.72、24.60±0.65、31.30±2.31；9.50±0.06、17.75±0.19、30.00±0.41、38.40±1.01；单位为1×104 个/孔，与对照组比较P<0.01）。联合用药时抑制作用最强的为CPT-11、DDP、5-FU联合用药组，后依次为CPT-11、DDP联合、CPT-11、 5-FU联合、5-FU、DDP联合（四组药物作用于相同起始浓度的肝癌细胞株48、72、96、120 h后细胞数量分别为6.30±0.79、9.50±0.66、14.50±0.03、21.40±0.66；6.60±0.54、12.20±0.71、20.90±0.45、28.80±0.36；9.00±0.61、16.40±0.29、30.40±0.37、37.30±0.25；12.00±0.02、18.80±0.39、30.70±0.07、35.40±0.08；单位为1×104 个/孔，与对照组比较P<0.01）。［结论］CPT-11对肝癌细胞株具有一定的细胞毒性作用，并强于目前肝癌临床常规用药5-FU、DDP，CPT-11可与DDP、5-FU联合应用于原发性肝癌的治疗。

Abstract:

［Objective］This study explore the cytotoxic effect of CPT-11 and combinated with 5-FU and DDP on hepatocellular carcinoma strain.［Methods］To detect the depression rate of CPT-11，5-FU and DDP on human hepatocellular carcinoma HepG2 strain respectively though MTT colorimetric method and to determine further the depression rate of combination of three drugs to contract.［Results］The growth of cell was depressed at various levels by sequential concentration of CPT-11,5-FU and DDP , and the effect promote with increasement of concentration; the depression effect of CPT-11 was most great in the three drugs, the more was DDP, and the last was 5-FU(the respective cell quantities of CPT-11 and DDP and 5-FU on human hepatocellular carcinoma strain with equal initial concentration after 48，72，96，120 h was 4.70±0.25，12.71±0.23，20.30±1.59，24.20±2.31；6.50±0.81，16.25±0.72，24.60±0.65，31.30±2.31；9.50±0.06，17.75±0.19，30.00±0.41，38.40±1.01；1×104/ hole，contrast with control group P<0.01), while combination with three drugs depress most strongly cell growth in various drugs combination, the more is CPT-11 and DDP,CPT-11 and 5-FU, the last was 5-FU and DDP (the respective cell quantities of four groups on human hepatocellular carcinoma strain with equal initial concentration after 48，72，96，120 h was:6.30±0.79，9.50±0.66，14.50±0.03，21.40±0.66；6.60±0.54，12.20±0.71，20.90±0.45，28.80±0.36；9.00±0.61，16.40±0.29，30.40±0.37，37.30±0.25；12.00±0.02，18.80±0.39，30.70±0.07，35.40±0.08；1×104/ hole，contrast with control group P<0.01).［Conclusion］There is considerable effect of CPT-11 on hepatocellular carcinoma strain. It is more obvious than 5-FU and DDP, which are used commonly in clinic. The strong depression of combination with three drugs provide theoretic foundation for clinical combination therapy.