乙型肝炎病毒前S1抗原与HBV-DNA和HBeAg的相关性分析

Analysis on detection results of pre S1-Ag ,HBV-DNA and HBeAg

关键词:  乙型肝炎前S1抗原  乙型肝炎病毒DNA  乙型肝炎e抗原

Key words:  HBV pre-S1 antigen  HBV-DNA  HBeAg

摘要:

［目的］探讨乙型肝炎病毒前S1抗原与乙型肝炎病毒DNA（HBV-DNA）、乙型肝炎病毒e抗原(HBeAg)的相关性。［方法］对207例乙型肝炎患者（HBV-DNA阳性）的血清用酶联免疫法（ELISA）进行前S1抗原、HBeAg、Anti-HBe、HBcIgG的检测。用PCR荧光定量法检测HBV-DNA，同时用ELISA法对乙型肝炎病毒血清标志物阴性的80例正常健康人也进行前S1抗原的检测。［结果］207例HBV-DNA阳性的血清中，前S1抗原在HBeAg阳性而Anti-HBe阴性的血清中阳性率为70.5%，在Anti-HBe阳性而HBeAg阴性中的阳性率为68.9%，两组经χ²检验，P>0.05差异无显著性意义。在HBV-DNA低拷贝数时，前S1抗原的阳性率占25.6%，HBeAg的阳性率占11.1%；在高拷贝数时，前S1抗原的阳性率占41.1%，HBeAg的阳性率占46.3%。在低拷贝数时，前S1抗原的阳性率明显高于HBeAg。［结论］前S1抗原、HBeAg与HBV-DNA的符合率较高，对无条件检测HBV-DNA而HBeAg阴性的血清检测前S1抗原，可提示乙型肝炎病毒处于低复制的状态，如与其他乙型肝炎病毒血清标志物同时检测，对临床更有意义。

Abstract:

［Objective］To discuss the duplication of hepatitis B virus (HBV) and suggest clinical diagnosis and treatment via comparison of correlation of HBV-DNA,HBeAg with HBV pre-S1 antigen.［Methods］For the 207 HBV-DNA positive patients,measure HBV pre-S1 antigen, HBeAg and Anti-HBe by ELISA and HBV-DNA by fluorescence quantitative PCR.［Results］In 207 HBV-DNA positive serum ,the positive rate of HBV pre-S1 antigen in HBV-DNA positive and HBeAg positive was 70.5%;in HBV-DNA positive and Anti-HBe positive was 68.9%.The difference of the two groups was not significant.In low copies of HBV-DNA(10³-10⁶ copies/ml),the positive rate of HBV pre-S1 antigen is 25.6%,HBeAg is 11.1%;in high copies of HBV-DNA,the positive rate of HBV pre-S1 is 41.1%,HBeAg is 46.3%.So in low copies,the positive of HBV pre S1 was higher than HBeAg.［Conclusions］HBV pre S1 antigen accompany with HBV-DNA. For the results of HBV-DNA positive and HBeAg negative, it is clinically significant to detect pre-S1 antigen in Hepatitis B immunological examination.