鹿茸对去卵巢小鼠子宫内膜LIF蛋白表达的影响

Effect of deer antler on expression of LIF protein in endometria of ovariectomized mice

关键词:  鹿茸；LIF  去卵巢小鼠

Key words:  deer antler  LIF  ovariectomized mice

摘要:

［目的］观察鹿茸对去卵巢(ovariectomized, OVX)小鼠子宫内膜白血病抑制因子（leukemia inhibit tory factor, LIF）蛋白的表达情况。［方法］雌性小鼠去卵巢10 d 后，随机分为对照组和实验组。实验组用鹿茸冻干粉每天以2 mg、6 mg和18 mg剂量连续给去卵巢小鼠灌胃14 d，对照组为仅用等量生理盐水，通过免疫组化染色和灰度值检测来确定子宫内膜LIF蛋白的表达情况。［结果］鹿茸处理去卵巢小鼠子宫内膜LIF蛋白的表达主要定位在子宫的腔上皮细胞和腺上皮细胞，通过灰度值检测得到：2 mg组、6 mg组、18 mg组及对照组分别是(144.78±3.46)、(131.37±3.83)、(113.80±7.04)和(193.10±4.49)。2 mg组、6 mg组和18 mg组与对照组比较均有统计学意义(P<0.05)。［结论］鹿茸促进去卵巢小鼠子宫内膜LIF蛋白的表达，并且随着鹿茸的剂量的增大而逐渐增加。

Abstract:

［Objective］To observe the effect of deer antler on the expression of LIF protein in endometria of ovariectomized mice.［Methods］Forty female mice were ovariectomized(OVX). After 10 days, those mice were randomly divided into experimental groups and control groups. The mice of three experimental group were divided into three subgroups and intragastricly injected with 2 mg, 6 mg and 18 mg of deer antler's low -free zed-dry powder respectively for 14 days, The mice of control group were intragastricly injected with normal saline for 14 days. The expression of LIF protein in endimetria was tested by immunohistochemistry staining and gray value.［Results］The LIF protein in endimetria is mainly secieted at luminal epithelium cells and glandular epithelium cells of uterus. The gray values of 2 mg group, 6 mg group，18 mg group and control group are (144.78±3.46), (131.37±3.83) , (113.80±7.04) and (193.10±4.49) respectively. Compared to the value of control group, the values of 2 mg group,6 mg group and 18 mg group are significantly increased, P<0.05.［Conclusions］Deer antler may promote expression of the LIF protein in endometria of ovariectomized mice, moreover, with increase in the dose of deer antler.