胃黏膜上皮错配修复基因hMLH1表达在胃癌诊断中的临床意义

Significances of hMLH1 protein expression in predication and diagnosis of gastric carcinoma

关键词:  错配修复基因  胃癌  hMLH1基因

Key words:  mismatch repair gene  gastric carcinoma  hMLH1 gene

摘要:

［目的］探讨hMLH1蛋白表达在胃癌诊断中的意义及用于胃癌预警的可能性。［方法］免疫组织化学（SP）法检测106例胃癌患者的癌（癌组）与癌旁黏膜上皮（癌旁组）的hMLH1蛋白表达情况并与34例非肿瘤患者（正常组）胃黏膜上皮进行比较，分析该蛋白在细胞中的表达部位与病变的关系。［结果］癌组和癌旁组及正常组hMLH1蛋白的胞核阳性表达率分别为71% (75/106)、50% (53/106) 和26% (9/34)，各组间差异有显著性意义（P<0.05）；胃癌组hMLH1蛋白在核、浆中同时表达阳性率为38% (40/106) 显著高于癌旁组和正常组的17% (18/106)和15%(5/34)（P<0.05），但后两组间差异无显著性意义。［结论］hMLH1蛋白在细胞核中出现高表达可能是胃黏膜上皮细胞癌变的组织学预警标志，而其在细胞核和细胞浆同时高表达对胃癌诊断具有一定意义。

Abstract:

［Objective］To study the possibility of hMLH1 protein as a marker to predict and diagnose gastric carcinoma.［Methods］The expression of hMLH1 protein was detected by immunohistochemisty (SP) in carcinomas（carcinoma group）and their surrounding epithelia (surrounding group) from 106 patients and gastric epithelia (normal group) from 34 non-carcinoma persons.［Results］The nucleus positive expression rates of hMLH1 protein in carcinoma, surrounding and normal group were 71% (75/106),50% (53/106) and 26% (9/34) respectively (P<0.05). Its positive expression rates of both nucleus and cytoplasma of carcinoma group was 38% (40/106), which was higher than those of surrounding group and normal group being 17% (18/106) and 15 %(5/34) (P<0.05).［Conclusions］The high nucleus expression of hMLH1 protein could predict gastric carcinogenesis, and the expressing protein in both nucleus and cyloplasma might be used as a marker to diagnose gastric carcinoma.