比色法测定大麻药及其提取物中总皂苷的含量

Determination content of total Saponins of dolichos tenuicaulis (Baker) Craib and crude extracts by spectrophotometry

关键词:  大麻药  总皂苷  比色法

Key words:  dolichos tenuicaulis (Baker) Craib  total saponins  spectrophotometry

摘要:

［目的］建立大麻药及其相关提取物的总皂苷含量分析方法。［方法］样品经香草醛－高氯酸显色后，以人参皂苷Rg1为对照品，进行紫外分光光度测定，优选最佳分析条件。［结果］最佳分析条件为：0.2 mL 5% 香草醛－冰醋酸溶液和0.8 mL高氯酸为显色剂，显色温度为60℃，显色时间为15 min，测定波长为550 nm。样品在3.25 ~ 19.52 μg/mL 范围内呈良好的线性关系，回归方程为 Y=0.030X+0.117（r=0.9995），平均加样回收率为97.45% （RSD=1.75%，n=5）。［结论］本法可作为大麻药及相关提取物中皂苷的质量控制方法。

Abstract:

［Objective］To establish a method for determination of total saponins of dolichos tenuicaulis (Baker) Craib and crude extracts by UV.［Methods］The samples were determined by UV after reaction with vanillin-perchloric acid, and ginsenoside Rg1 was selected as the standard. Some parameters which might effect the results were all investigated and optimized.［Results］The suitable analytic conditions were optimized as follows: 5% vanillin- acetic acid solution 0.2 mL, perchloric acid 0.8 mL, temperature 60℃, reaction time 15 min.，detection wavelength 550 nm. The samples were linear in the range of 3.25~19.52 μg/mL with the regression equation of Y=0.030X+0.117(r=0.9995), and the average recovery was 97.45% (RSD=1.75%, n=5) under the selected conditions.［Conclusions］This method is suitable for the determination of total saponins for quality control of dolichos tenuicaulis (Baker) Craib.