参杞合剂抗肿瘤免疫的动态实验研究

Effect of naloxone on spacial working memory of chronic ethanol intake in rats

关键词:  纳洛酮  酒精  空间工作记忆  Morris水迷宫

Key words:  naloxone  ethanol  spatial working memory  morris water waze

摘要:

［目的］ 研究阿片受体拮抗剂纳洛酮对慢性酒精中毒大鼠空间工作记忆的影响。［方法］SD大鼠30只随机均分成3组，即:饮酒/生理盐水组、饮酒/纳洛酮组、饮水/生理盐水组。给饮酒/生理盐水组和饮酒/纳洛酮组大鼠自由饮用由含6％逐渐递增至15%（v/v）的乙醇水溶液8周,建立慢性酒精中毒模型，8周后饮酒/纳洛酮组连续10 d腹腔注射纳洛酮，其他组动物腹腔注射相同体积生理盐水，然后对各组动物进行Morris水迷宫训练。［结果］3组大鼠水迷宫训练后trails 2的游泳路径长度分别为(481.35±183.78)cm、(379.54±221.10)cm和(302.76±210.47)cm，与trails 1比较，差异有显著性意义(P<0.05)；其前后两次游泳路径的平均savings ratio值饮酒/生理盐水组为0.35±0.08，与饮水/生理盐水组0.59±0.09比较，差异有显著性意义(P<0.01)；饮酒/纳洛酮组经纳洛酮处理后savings ratio 为0.55±0.10,明显高于饮酒/生理盐水组(P<0.05)。［结论］长期酒精摄入后会损害大鼠的空间工作记忆能力，纳洛酮能够减轻这种损害，说明阿片系统参与了空间工作记忆的过程。

Abstract:

［Objective］To study the effect of naloxone on spatial working memory of chronic ethanol intake(CEI) in rats. ［Methods］Alcohol-induced spatial working memory impairment model was established by 8 weeks continuous alcohol consumption. The rats with ethanol were randomly divided into two groups including the alcohol-treated group and the alcohol/naloxone-treated group which was treated viz. naloxone injection(i.p.) for 10 days, and the non-treated group. The alcohol-treated group and the non-treated group received the same volume saline instead of naxolone. All rats were trained in morris water maze.［Results］The mean swimming paths distance savings ratio of two trails in rats of the alcohol-treated group 0.35±0.08 was significantly decreased compared with that of the non-treated group0.59±0.09 (P<0.01),while it was significantly increased in that of the alcohol/naloxone-treated group 0.55±0.10 compared with the alcohol-treated group (P<0.05). ［Conclusions］Naloxone can ameliorate alcohol-induced spatial working memory impairment in rats, suggesting that the opiate system may be involved in the processing of working memory.