乙胺丁醇处理前后耻垢分枝杆菌mc2155细胞蛋白双向电泳图谱的差异分析

Simultaneous detection of proteins expression of Mycobacterium smegmatis mc2155 after treatments of ethambutol (EMB) by two-dimensional electrophoresis

关键词:  乙胺丁醇  耻垢分枝杆菌 mc²155  双向电泳  蛋白质组比较

Key words:  ethambutol (EMB)  Mycobacterium smegmatis mc²155  two-dimensional gel electrophoresis (2-DE)

摘要:

［目的］利用蛋白质组学方法，识别乙胺丁醇处理前后耻垢分枝杆菌mc²155细胞后差异表达的蛋白质，为进一步探讨乙胺丁醇抑制分枝杆菌生长的作用机制及寻找新的抗结核药物作用靶标奠定基础。［方法］采用载体两性电解质pH梯度双向凝胶电泳技术分离乙胺丁醇处理前后耻垢分枝杆菌mc²155细胞的总蛋白质，用PDQuest 7.3.1图像分析软件比较分析，以识别差异表达的蛋白质。［结果］空白对照组有268个斑点，Ethambutol（EMB）处理组有蛋白斑点195个，在相对分子质量和pI值分布的任一区域，EMB处理组蛋白斑点数均少于空白对照组，其中在EMB处理组中特异表达的蛋白质斑点有23个。［结论］乙胺丁醇处理前后耻垢分枝杆菌mc²155细胞的蛋白质组具有差异，这种蛋白质组的差异分析有助于进一步研究乙胺丁醇抑制分枝杆菌生长的作用机制并为寻找新的抗结核药物作用靶标奠定基础。

Abstract:

［Objective］To investigate the microbiostatic mechanism of ethambutol (EMB), proteomic methods were used to find differential expression proteins in Mycobacterium smegmatis mc²155 cells treated with ethambutol. ［Methods］The total proteins of untreated and ethambutol treated mc²155 cells were separated by ampholyte pH gradients based two-dimensional gel electrophoresis (2-DE). The differential expression proteins were analyzed by using image analysis software. ［Results］ Proteins of control group and EMB-treated group contained 268 and 195 protein spots, respectively. Both in relative molecular weight range and in pI , EMB-treated group protein spots are less than those of control group. ［Conclusions］There is a significant difference at protein level between untreated and ethambutol treated mc²155 cells. It suggests that the differential expression analysis of proteomes may be useful for further study on microbiostatic mechanism of ethambutol and search for new targets toward anti-TB drug development.