银杏叶提取物对脂多糖介导的血管内皮细胞低密度脂蛋白受体 LOX-1 表达的影响

Effect of GBE50 on LPS-induced LOX-1 expression in HUVECs

关键词:  脐静脉内皮细胞  银杏叶提取物（GBE50）  氧化性低密度脂蛋白受体（LOX-1）

Key words:  HUVECs  GBE50  LOX-1

摘要:

［目的］ 观察银杏叶提取物（GBE50）对脂多糖（LPS）诱导的人脐静脉内皮细胞（HUVECs）氧化低密度脂蛋白受体（lectin like oxidized low density lipoprotein receptor，LOX -1）表达的影响。［方法］应用LPS刺激体外培养的HUVECs 24h。RT-PCR方法检测（lectin like oxidized low density lipoprotein receptor mRNA，LOX-1 mRNA）表达水平；采用蛋白质印迹分析检测LOX-1蛋白表达水平。［结果］LPS (1 μg/ mL)上调LOX-1 mRNA和蛋白表达水平，GBE50（40 μg/mL）轻度抑制LPS介导的LOX-1 mRNA（P<0.005）和蛋白表达增加（但没有统计学意义，P>0.05），GBE50（80 μg/mL）及NF-κB抑制剂CAPE（20 μg/mL）明显抑制LOX-1 mRNA和蛋白表达上调（P<0.01）。［结论］ GBE50可能通过抑制LOX-1表达，在防治动脉粥样硬化中发挥作用。

Abstract:

［Objective］ To investigate the effect of Ginkgo biloba extract 50 (GBE50) on LPS-induced LOX-1 expression in HUVECs.［ Metheod］ HUVECs were incubated by purified LPS for 24 h. LOX-1 mRNA were measured by RT-PCR, the protein expression of LOX-1 were detected by Western blot. ［Results］ LPS(1 μg/mL) upregulated LOX-1 mRNA and protein expression, GBE50（40 μg/mL）suppressed LPS-induced LOX-1 mRNA expression(P<0.05) and protein expression(P<0.05) GBE50（80 μg/mL）and NF-κB inhibitor CAPE both significantly inhibited LOX-1 mRNA expression and protein expression upregulation. ［Conclusion］ GBE50 may play a role in anti-atherosclerosis by downregulating LOX-1 expression.