一氧化氮在大剂量顺铂所致大鼠肾损伤早期中的变化

Function of nitric oxide in high dose cisplatin induced acute renal failure in rats

关键词:  大剂量顺铂  肾功能衰竭  SD大鼠  动物模型  一氧化氮(NO)

Key words:  high-dose of cisplatin (DDP)  acute renal failure  SD rats  animal model

摘要:

［目的］观察一氧化氮(NO)在大剂量顺铂所致大鼠急性肾功能衰竭早期中的变化。［方法］ SD大鼠18只，雌雄各半，随机分为顺铂（DDP）用药组和生理盐水对照组，每组9只。各组腹腔内一次性注射顺铂10 mg/kg或等量生理盐水后，动态观察大鼠体重的变化，用药后6 h取各组肾脏计算肾系数，并用生化法测定血清尿素氮和肾皮质匀浆中NO的水平，统计学分析。［结果］ 大剂量顺铂用药6 h后，大鼠体重开始明显降低，用药后3 d大鼠死亡。用药后6 h血清BUN水平两组比较无显著差异（ P>0.05），顺铂用药组为(18.41±4.7)mg/dL，对照组为(18.44±4.03)mg/dL；但用药后6 h肾系数和肾皮质匀浆NO水平两组比较差异显著(P<0.05)，顺铂用药组分别为(9.39±0.78)和(24.79±11.27) μmoL/gprot，对照组分别为(8.34±0.60)和（5.27±2.4）μmoL/gprot。［结论］ 10 mg/kg顺铂可引起大鼠急性肾功能衰竭并致死，NO在肾损伤早期发挥重要作用。

Abstract:

［Objective］To investigate the level of nitric oxide (NO) in high dose cisplatin induced acute renal failure in rats. ［Methods］ SD rats were received cisplatin (10 mg/kg) intraperitoneally and the same volume of natural saline was given as control. Body weight of rats was measured before and 6 h, 48 h, 72 h after the administrations. Renal coefficient was measured 6 h after the injection. The levels of blood urea nitrogen (BUN), protein contents and NO contents in renal cortex tissue were measured 6 hours after administrations. ［Results］ Body weight of rats decreased significantly after cisplatin administration and the rats died within 3 d. There are no significant differences in BUN between cisplatin group (18.41±4.7)mg/dL and control group(18.44±4.03)mg/dL 6 hours after administrations （P> 0.05）. But the renal coefficient and the levels of NO in renal cortex tissue are (9.39±0.78) and (24.79±11.27) μmoL/gprot respectively in cisplatin group, significantly higher than those (8.34±0.60) and （5.27±2.4）μmoL/gprot respectively in control group 6 hours after administrations (P<0.05). ［Conclusions］ Acute renal failure induced by high dose of cisplatin (10 mg/kg) can cause rats dead. NO plays an important role in the early stage of acute renal failure.