PTEN 和hMSH2蛋白的表达在胃癌发生中的作用

Roles of PTEN and hMSH2 expressions in gastric carcinogenesis

关键词:  抑癌基因  PTEN蛋白  错配修复基因  hMSH2蛋白  胃癌

Key words:  tumor suppressor gene  PTEN protein  mismatch repair gene  hMSH2 protein  gastric carcinoma

摘要:

［目的］通过探讨胃癌组织PTEN 和hMSH2蛋白的表达的相互关系，分析二者表达在胃癌发生中的意义。［方法］ 用链霉素-生物素蛋白-过氧化酶法(SP法)检测63例胃癌组织及32例非癌胃黏膜组织中PTEN 和hMSH2蛋白的表达情况。［结果］ PTEN蛋白在胃癌组织中的阳性表达率（61.9%）（39/63）显著低于非癌胃黏膜组织（90.6%）（29/32）（P<0.05）,在高中分化腺癌中的阳性表达率（57.9%）（11／19）低于低分化腺癌（72.4%）（21／29）和黏液癌（60%）（9／15），但无显著性差异（P>0.05）；hMSH2蛋白在胃癌组织中的阳性表达率（79.4%）（50／63）显著高于非癌胃黏膜组织（53.1%）（17／32）（P<0.05）,但在高中分化腺癌（73.7%）（14／19）、低分化腺癌（82.8%）（24／29）和黏液癌（80%）（12／15）中的阳性表达率无显著性差异（P>0.05）。PTEN和hMSH2蛋白在胃癌组织中的表达无明显相关关系（r＝－0.158，P>0.05）。［结论］ 胃癌发生时可出现PTEN蛋白表达下调，伴hMSH2蛋白的表达上调。

Abstract:

［Objective］ To examine the expressions of PTEN and hMSH2 in gastric carcinoma and study the roles of their expressions in gastric carcinogenesis. ［Method］Streptavidin-biotion-peroxidase method (SP) was used to detect the expressions of PTEN and hMSH2 in gastric carcinoma and normal mucosa as control. ［Results］ The positive rate of PTEN expression in gastric carcinoma being 61.9% (39/63) was significantly lower than that in gastric normal mucosa being 90.6% (29/32)(P<0.05), and the rate in well & middle differentiated group being 57.9%(11/19) was lower than those in poor differentiated group being 72.4%(21/29) and mucoid carcinoma group being 60%(9/15), but there was no significant difference between them (P>0.05). The positive rate of hMSH2 expression in gastric carcinoma being 79.4% (50/63) was significantly higher than that in gastric normal mucosa being 53.1%(17/32)(P<0.05). The expression of hMSH2 was not related to the differentiation of gastric carcinoma (P>0.05). The expressions of PTEN were not related to the expressions of hMSH2 in gastric carcinoma and normal mucosa. ［Conclusion］ The absence of PTEN expression with the up-regulated hMSH2 expression related to gastric carcinogenesis. It may be helpful for diagnosing gastric carcinoma to detect the expressions of the two gene products.