院内感染洋葱伯克霍尔德菌的分析

Analysis of burkholderid depacia in nosocomial infection

关键词:  洋葱伯克霍尔德菌  耐药率  抗生素

Key words:  burkholderid depacia  drug resistance rate  antibiotics

摘要:

［目的］ 回顾大连医科大学第一临床学院2004年送检的各种标本中洋葱伯克霍尔德(burkholderiddepacia，BC)菌的分离与药敏结果，为院内感染洋葱伯克霍尔德菌的治疗提供可靠的依据。［方法］菌种的鉴定采用法国生物一梅里埃公司的API2ONE鉴定系统，对分离到的36株洋葱伯克霍尔德菌进行准确鉴定，药敏试验采用K—B琼脂扩散法，培养基的制备和药敏试验操作均严格按照NCCLS的规定执行。［结果］ 36株洋葱伯克霍尔德菌分别分离于痰标本、导管尖端、尿液和血标本中。其中痰标本31株，导管尖端2株，尿标本2株、1株血标本。药敏结果如下：耐药率最高为头孢西丁（94.4%）其次是庆大霉素(83.3％)，阿米卡星(66.7％)，敏感率较高的有头孢哌酮／舒巴坦(88.9％)，头孢他啶(77.8％)， 环丙沙星(75％)。［结论］院内感染的洋葱伯克霍尔德菌主要分离于痰标本，治疗首选抗生素为头孢哌酮/舒巴坦。

Abstract:

［Objective］ To review the Burkholderid depacia isolated in our hospital in 2004, providing foundation for clinical therapy of Burkholderid depacia in nosocomial infection. ［Methods］ Identification of 36 strains of BC was performed by API 20NE analysis system of the biomerieux company. The K-B agar diffusion test according to NCCLS was used.［Results］ 36 strains of BC wereisolated from sputum、catheter tip、urine and blood specimens. Among the total strains, 31 strains were isolated from sputum specimens, 2 strains were isolated from catheter tip specimens, 2 strains were isolated from urine specimens, 1 strain was isolated from blood specimen. The antimicrobial susceptibility test result: The highest resistance rate was cefoxintin (94.4%), then were gentamicin (83.3%), a mikacia(66.7%); the stronger susceptibility rates werecefoperazone/sulbactam(88.9 %),ceftazidime(77.8%),ciprofloxacin(75%).［Conclusion］ Most of Burkholderid depacia in nosocomial infection were isolated from sputum specimens.The best antibiotics is cefoperazone/sulbactam.