哮喘小鼠血清IL-4和IL-15相关性分析

Analysis serum interleukin-4 and interleukin-15 levels of asthma model

关键词:  支气管哮喘  IL-4  IL-15  地塞米松

Key words:  Asthma  Interleukin-15  Interleukin-4  dexamethasone.

摘要:

［目的］通过对哮喘小鼠模型血清中白细胞介素15（IL-15）与IL-4水平进行分析，探讨其二者之间的相关性。 ［方法］ BALB/c小鼠随机分为3 组, 分别为正常对组、哮喘组、地塞米松干预组。用鸡卵白蛋白(OVA) 抗原溶液腹腔注射致敏,雾化吸入激发制备哮喘小鼠模型。对照组用生理盐水代替OVA；干预组每次雾化前腹腔注射地塞米松进行干预。定雾化激发后24 h取各组肺泡灌洗液进行嗜酸性细胞计数，并用ELISA方法测测定血清IL-15、IL-4的水平,分析二者相关性。 ［结果］IL-4与IL-15水平和嗜酸性细胞数各组比较均有显著性差异(P<0.01)，哮喘组分别为(18.73±3.07 )pg/mL，(18.10±2.66) pg/mL和（9.57±1. 63)×10⁵/L；干预组分别为(14.04±1.58)pg/mL， (14.37±2.46) pg/mL和（7.47 ±2.11)×10⁵/L；对照组分别为(9.26±1.87)pg/mL，(10.82±2.13) pg/mL和（ 3.22±1.78×105/L)；血清IL-4与IL-15水平呈正相关，r=0．57，P<0．05。［结论 ］IL-4与IL-15在哮喘发病中有一定的联系，IL-4作为一种对哮喘发生发展起负向调节作用的细胞因子可以推断IL-15在哮喘中起到一定的调节作用。

Abstract:

［Objective］To investigate the levels of serum interleukin-15（IL-15）and interleukin-4(IL-4) in an asthma mouse model, and analysis the associativity between them. ［Methods］ Mice were randomly divided into three groups：Asthma group(group A)，dexamethasone group(group B)，and normal control group (group C)．Mice in group A and B were sensitized by intraperitoneal injections of OVA and Al(OH)3 on days 0 and 14. On days 18, 19, and 20, animals were challenged with 10g/L OVA aerosol for 20 min. Mice in group B were intraperitoneal injections of dexamethasone before challenged with OVA . Mice in group C were sensitized and challenged with saline instead. To determine the serum interleukin-15 and interleukin-4 levels at 24 h after challenge with the enzyme linked immunosorbent assay ；and count the eosinophil cells in bronchoalveolar lavage fluid. ［Restlts］ The results show that the levels of IL-15, IL-4 and eosinophil cells in Group A ( 18.733.07) pg/mL,(18.102.66) pg/mL and (9.571.63)×105/L are significantly higher than those in Group C(P<0.01) (9.261.87) pg/mL,(10.822.13) pg/mL and (3.221.78)×10⁵/L and Group B(14.041.58) pg/mL,(14.372.46 )pg/mL and (7.472.11) ×10⁵/L. The correlation between the level of IL-15 and the IL-4 level was positive(r=0．57，P<0．05). ［Conclusion］IL-15 might play a role in the pathogenesis of asthma based on that IL-4 has the inhibition of asthma.