| 摘要: |
| ［目的］ 观察不同葡萄糖浓度环境、氟伐他汀对人系膜细胞表达FN、IV型胶原作用。［方法］ 培养人肾小球系膜细胞，分为正常葡萄糖组、高葡萄糖组及加氟伐他汀组，培养24、48 h收集细胞上清液，用酶联免疫法检测FN、IV型胶原。［结果］ 在高糖组24、48 h FN分别是(1.552±0.546)和(2.547 0±0.968) ng/mL，明显高于正常糖组24 、48 h，FN:(0.777±0.076)和(1.180±0.091) ng/mL（P<0.01）； 在高糖组24 、48 h IV型胶原分别是(64.577±5.543)和(80.140±6.605) ng/mL，明显高于正常糖组24、48 h IV型胶原：(42.468±5.615)和(46.368±5.714) ng/mL（P<0.01） 。氟伐他汀可减少高糖浓度下细胞上清液中FN、Ⅳ型胶原的表达水平。在高糖组与氟伐他汀
(1 μmol/L,10 μmol/L)作用24、48 h FN和IV型胶原分别是(0.955±0.102)、(0.858±0.081) ng/mL，(0.652±0.078)、(0.822±0.257) ng/mL（P<0.01）、(34.908±4.283)、(32.163±2.220)、(28.260±4.287)、(23.233±7.284) ng/mL（P<0.01）。［结论］ 氟伐他汀可抑制高糖刺激的系膜细胞表达FN、Ⅳ型胶原。 |
| 关键词: 氟伐他汀 系膜细胞 基质 |
| DOI：10.11724/jdmu.2006.04.09 |
| 分类号:R587.1 |
| 基金项目: |
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| Effect of fluvastatin on production of extracellular matrix in glomerular mesangial cell |
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YIN Yong-hong1, Lü Shen2
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1.Department of Nephrology,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China;2.Laboratory Center,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China
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| Abstract: |
| ［Objective］ To observe the effect of glucose concentration,fluvastatin on human mesangial cells to produce FN and collagen Ⅳ. ［Methods］ Human mesangial cells were cultured . Then all cultured cells were divided into normal glucose group、high glucose group and experimental groups . Human mesangial cells were incubated with different glucose (5 or 25 mmol/L) with or without different concentrations of fluvastatin for 48 hours. Type Ⅳcollagen and fibronectin (FN ) levels were assessed by ELISA. ［Results］ Human glomerular mesangial cells in different glucose expressed FN and type Ⅳ collagen. Fluvastatin decreased the level of cell culture supernatant of FN and type Ⅳ collagen in high glucose. The levels of FN in 25 mmol/L glucose at 24 or 48 hours being (1.552±0.546) and (2.547±0.968) ng/mL were significantly higher than those in 5.0 mmo/lL glucose being (0.777±0.076) and (1.180±0.091) ng/mL (P<0.01). The levels type Ⅳ collagen of in 25 mmol//L glucose at 24 or 48 hours being (64.577±5.543) ng/mL and (80.140±6.605) ng/mL were significantly higher than those in 5.0 mmol/L glucose being (42.468±5.616) and (46.368±5.714) ng/mL (P<0.01). The levels of FN and type Ⅳ collagen in 25 mmol/L glucose group at 24 or 48 hours were (0.955±0.102) or (0.858± 0.081) ng/mL,(0.652±0.078) or (0.822±0.257) (P<0.01) and (34.908±4.283) or (32.163±2.220) ng/mL,(28.260±4.287) or (23.233±7.284) (P<0.01) respectively. ［Conclusion］ Fluvastatin decreased expression of FN and type Ⅳ collagen in cultured mesangial cell by high glucose stimulation. |
| Key words: fluvastatin mesangial cell matrix |
