胰腺癌组织中HSP60、HSP70和P53的表达及意义

Expression and significance of HSP60，HSP70 and P53 in pancreatic carcinoma

关键词:  热休克蛋白  P53  胰腺癌  免疫组织化学

Key words:  heat shock proteins  P53  immunohistochemistry  pancreatic cancer

摘要:

［目的］ 了解HSP60、HSP70及P53在慢性胰腺炎组织及胰腺癌组织中的表达及其意义。［方法］ 应用免疫组织化学ABC法检测HSP60、HSP70及P53在慢性胰腺炎12例、癌旁组织及胰腺癌63例（其中高、中、低分化各为14、23、26例）中的表达，结果进行卡方检验及相关性分析。 ［结果］ 在慢性胰腺炎、癌旁组织及胰腺癌中，HSP60的过表达率17%、10%、70%（P<0.01）；HSP70的过表达率25%、13%、75%（P<0.01）。在高、中、低分化胰腺癌中，HSP60的过表达率43%、70%、85%（P>0.05〉; HSP70的过表达率36%、78%、92%(P<0.01)。HSP70的过表达率与是否发生淋巴结转移有显著关系（P<0.05）；P53在胰腺癌中的表达率59%，与HSP60、HSP70在胰腺癌中的表达呈正相关（P<0.01）。［结论］ 胰腺癌是HSP60、HSP70高表达肿瘤，HSP70与胰腺癌临床病理特征和生物学行为有更密切的关系。P53与HSP60、HSP70在肿瘤中的表达密切相关。

Abstract:

［Objective］ The experiment discovered the expression and significance of HSP60，HSP70，P53 in chronic pancreatitis and pancreatic cancer. ［Methods］Immunohistochemical staining avidin-biotin complex was used to detect the expression of HSP60，HSP70，P53 in specimens of 12 cases of chronic pancreatitis，63 cases of tumor-surrounding tissues and pancreatic cancer(the amount of high differetiation were 14 cases,middle 23 cases,low 26 cases). The result was analyzed in chi-square test and correlation test. ［Result］ In chronic pancreatitis，tumor-surrounding tissues and pancreatic cancer,the overexpression rate of HSP60 was 17%，10%， 70%(P<0.01);The overexpression rate of HSP70 was 25%，13%，75%(P<0.01)。In the high，middle，low differetiational pancreatic cancer, the overexpression rate of HSP60 was 43%,70%，85%（P>0.05）; the overexpression rate of HSP70 was 36%,78%，92%（P<0.01）.The overexpression of HSP70 showed significant difference between groups with lymph node metastasis and without（P<0.05）；The expression rate of P53 in pancreatic cancer tissues was 59%，correlated with the expression of HSP60 and HSP70 in pancreatic cancer（P<0.01）.［Conclusion］ Pancreatic carcinoma is the tumor of high expression of HSP60 and HSP70.HSP70 had tight relation withclinic-pathological character and biological behaviors of pancreatic carcinoma.P53 and HSP60 and HSP70 had tight relation in the expression of pancreatic cancer