胃癌细胞 p27kip1 、cyclinE 的表达及与幽门螺杆菌感染关系

Expressions of p27kip1 and cyclinE in gastric cancer and their relationship to helicobacter pylori infection

关键词:  胃癌  幽门螺杆菌  p27^kip1  cyclinE

Key words:  gastric carcinoma  helicobacter pylori （Hp）  p27^kip1  cyclinE

摘要:

［目的］观察胃癌细胞p27^kip1、cyclinE的表达情况，并分析幽门螺杆菌(Helicobacter pylori ,Hp)感染对二者表达的影响,以探讨Hp可能的致癌机制。 ［方法］ 用免疫组织化学SP法检测59例胃癌p27^kip1、cyclinE表达情况，采用改良Giemsa快速尿素酶检验法检测Hp感染。［结果］ p27^kip1阳性率在正常胃黏膜为84.22%，显著高于胃癌的47.46%（P<0.05）,胃癌中高中分化腺癌组为68.42%，显著高于低分化腺癌的37.5%和与胃黏液癌的31.25%（P<0.05），但后两者间无显著性差异(P>0.05)。 cyclinE阳性率在正常胃粘膜为11.11%，显著低于胃癌的55.93%（P<0.05），胃癌中高中分化腺癌组为31.58%显著低于低分化腺癌的66.67%和胃黏液癌的68.75%（P<0.05），后两者间无显著性差异（P>0.05）。p27^kip1及cyclinE的阳性率在Hp阳性和Hp阴性胃癌组间无显著差异性（P>0.05）。 ［结论］胃癌组织p27^kip1表达下调、cyclinE表达上调，提示二者的表达失衡可能会促进胃癌的发生。但二者表达与Hp感染无明显相关关系。

Abstract:

［Objective］ To investigate p27^kip1and cyclinE gene product expressions of carcinoma cells and the relationship of the expressions to Helicobacter pylori (Hp) infection in gastric carcinoma. ［Methods］ The expressions of p27^kip1 and cyclinE in 59 gastric carcinomas were detected by immunohistochemical method (SP method) and Hp was examined by Giemsa staining and rapid urease test. ［Results］ The p27^kip1 expression rate of normal gastric mucosa being 84.22% was significantly higher than that of gastric carcinoma being 47.46% (P<0.05); the rate of well- middle differentiative carcinomas being 68.42% was significantly higher than those of poor differentiative carcinoma being 37.50% and mucoid carcinoma being 31.25% (P<0.05) . The cyclinE expression rate of normal gastric mucosa being 11.11% was significantly lower than that of gastric carcinoma being 55.93% (P<0.05); the rate of well-middle differentiative carcinomas being 31.58% was significantly lower than those of poor differentiative carcinoma being 66.67% and mucoid carcinoma being 68.75% (P<0.05).In gastric carcinoma, there was no significant relationship of the expressions of p27^kip1 and cyclinE to Hp infection (P>0.05). ［Conclusion］ The lower expression of p27^kip1 and the higher expression of cyclinE are associated with the development and the progression of gastric cancer, and there was no singificant association of the expression of p27^kip1/cyclinE with Hp infection.