| 摘要: |
| ［目的］ 探讨16S-23S rRNA间区基因RFLP用于临床常见病原菌区分的可能性。［方法］ 183株临床分离来的细菌经荧光PCR扩增后进行不完全限制性酶切，产物进行毛细管电泳。［结果］ 所有细菌的酶切产物经毛细管电泳后，不同细菌产生不同的RFLP谱图，互相之间能够明显区分。［结论］ 该方法可以将临床常见病原菌准确区分到种的程度，极有应用价值。 |
| 关键词: 16S-23S rRNA间区基因 病原菌 聚合酶链反应（PCR） 限制性酶切片段长度多态性（RFLP） |
| DOI：10.11724/jdmu.2005.04.08 |
| 分类号:R446.1 |
| 基金项目: |
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| Pathogenic bacteria differentiation by 16S-23S rRNA RFLP |
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WEN Jie1, LIU Yi2
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1.Dalian Municipal Centeral Hospital，Dalian 116033，China;2.Dalian Center of Clinical Laboratory，Dalian 116033，China
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| Abstract: |
| ［Objective］ The differentation possibility of 16S-23S rRNA spacer reg ion will be evaluated in this study. ［Method］ Total 183 strains collected from c linical patients were amplified by fluorescence-PCR and then digested by restric tion endonuclease Hae III incompletely. ［Result］ All the bacteria could be ide nt ified as definitely as the spices level. Different bacteria gave different RFLP patterns. ［Conclusion］ The method used in this study was a valuable alternativ e to the bacterial differentiation for clinical use. |
| Key words: 16S-23S rRNA spacer region gene polymerase chain re action pathogenic bacteria RFLP |
