| 摘要: |
| ［目的］ 了解16S rRNA基因在细菌区分中的应用价值。［方法］ 细菌热裂解后用荧光（FAM，JOE）标记引物进行扩增，产物用Hae III消化并进行毛细管电泳。［结果］ 所有细菌PCR产物均产生255bp片段，酶切产物电泳后具有科间差别。［结论］ 该方法可以用于不同细菌之间的分科。 |
| 关键词: 16S rRNA基因 病原菌 聚合酶链反应（PCR） 限制性酶切片段长度多态性（RFLP） |
| DOI：10.11724/jdmu.2005.03.29 |
| 分类号:R446.1 |
| 基金项目: |
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| Bacterial families classification by RFLP |
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WEN Jie1, LIU Yi2
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1.Dalian Municipal Centeral Hospital, Dalian 116033，China;2.Dalian Center of Clinical Laboratory, Dalian 116033, China
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| Abstract: |
| ［Objective］ In order to appraise the utility of 16S rRNA gene for the differentiation of different bacteria, a pair of primers were selected to amplify this gene. The PCR products were then digested by restriction endonuclease. ［Method］ All the bacteria were lysed by heat and amplified by two kinds of fluorescence -FAM and JOE, respectively, labeled primers. The PCR product were digested by Hae III and then taken capillary electrophoresis. ［Result］ All the bacteria can generated a 255bp product after amplification. The products digested were different in different families. ［Conclusion］ This method can be applied to the practice of differentiation of different bacteria belonging to different families. |
| Key words: 16S rRNA gene polymerase chain reaction pathogenic bacteria RFLP |
